Crosslinked urethane doped polyester biphasic scaffolds: Potential for in vivo vascular tissue engineering.
Crosslinked urethane doped polyester biphasic scaffolds: Potential for in vivo vascular tissue engineering.
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DOI：
10.1002/jbm.a.32846
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发表时间：
2010-11
影响因子：
4.9
通讯作者：
Yang, Jian
中科院分区：
文献类型：
--
作者：
Dey, Jagannath;Xu, Hao;Nguyen, Kytai Truong;Yang, Jian
活体组织工程将人体作为组织再生的生物反应器，因此对组织支架提出了严格的要求，组织支架应具有机械坚固性，可以立即植入，而无需长时间的体外细胞培养。除了机械强度外，采用活体组织工程方法制备的血管移植物还必须具有与目标组织相匹配的机械性能，以避免顺应性不匹配，这是移植物失败的原因之一。我们最近合成了新一代强弹性可生物降解的交联聚氨酯掺杂聚酯（CUPE），以解决开发柔软，弹性和强可生物降解聚合物的挑战。本研究评估了CUPE双相支架的抗拉强度、破裂压力和缝线保留度，以确定该支架是否符合活体组织工程方法的立即植入要求。此外，我们还检查了CUPE的血液相容性和炎症潜力，以证明其作为血液接触血管移植物材料的潜力。CUPE双相支架的抗拉强度（5.02±0.70 MPa）高于原生血管（1.43±0.60 MPa）。CUPE支架的破裂压力可调，范围从1500 mmHg到2600 mmHg，缝线保持值（2.45±0.23 N）合适。CUPE表现出与聚l -乳酸PLLA相当的白细胞活化和全血凝血动力学。然而，CUPE刺激炎症细胞因子的释放较少，并且被发现是非溶血的。结合力学性能和先前证明的抗血栓形成性质，CUPE可以作为活体血管组织工程的可行移植物材料。
In vivo tissue engineering uses the body as a bioreactor for tissue regeneration, thus placing stringent requirements on tissue scaffolds which should be mechanically robust for immediate implantation without a long in vitro cell culture time. In addition to mechanical strength, vascular grafts fabricated for in vivo tissue engineering approach must have matching mechanical properties to the target tissues to avoid compliance mismatch, which is one of the reasons for graft failure. We recently synthesized a new generation of strong and elastic biodegradable crosslinked urethane-doped polyesters (CUPE) to address the challenge of developing soft, elastic yet strong biodegradable polymers. This study evaluated the tensile strength, burst pressure and suture retention of CUPE biphasic scaffolds to determine if the scaffolds met the requirements for immediate implantation in an in vivo tissue engineering approach. Additionally, we also examined the hemocompatibility and inflammatory potential of CUPE to demonstrate its potential in serving as a blood-contacting vascular graft material. Tensile strength of CUPE biphasic scaffolds (5.02±0.70 MPa) was greater than native vessels (1.43±0.60 MPa). CUPE scaffolds exhibited tunable burst pressure ranging from 1500 mmHg to 2600 mmHg, and adequate suture retention values (2.45±0.23 N). CUPE showed comparable leukocyte activation and whole blood clotting kinetics to poly(L-lactic acid) PLLA. However, CUPE incited a lesser release of inflammatory cytokines and was found to be non hemolytic. Combined with the mechanical properties and previously demonstrated anti-thrombogenic nature, CUPE may serve as a viable graft material for in vivo blood vessel tissue engineering.
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影响因子：
4.8
作者：
L'Heureux, N;Pâquet, S;Auger, FA
通讯作者：
Auger, FA
DOI：
10.1115/1.429652
发表时间：
2000-06-01
影响因子：
1.7
作者：
Girton, TS;Oegema, TR;Tranquillo, RT
通讯作者：
Tranquillo, RT
影响因子：
82.9
作者：
Kaushal, S;Amiel, GE;Mayer, JE
通讯作者：
Mayer, JE
影响因子：
4.3
作者：
BASSIOUNY, HS;WHITE, S;ZARINS, CK
通讯作者：
ZARINS, CK
影响因子：
3.4
作者：
Hoerstrup, SP;Zünd, G;Turina, MI
通讯作者：
Turina, MI