Simulating the growth of articular cartilage explants in a permeation bioreactor to aid in experimental protocol design.
Simulating the growth of articular cartilage explants in a permeation bioreactor to aid in experimental protocol design.
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DOI：
10.1115/1.3049856
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发表时间：
2009-04
期刊：
影响因子：
--
通讯作者：
Klisch SM
中科院分区：
文献类型：
--
作者：
Ficklin TP;Davol A;Klisch SM
最近开发了软骨生长有限元模型（CGFEM）来解决非均匀且随时间变化的生长边值问题。 CGFEM 允许固体基质、胶原蛋白和蛋白聚糖的主要成分具有不同的应力本构方程和生长定律。当前工作的目的是在稳态渗透生物反应器中模拟关节软骨外植体的体外生长，以获得有助于实验设计的结果。稳态渗透协议会引起不同类型的机械刺激：当样品最初是均匀的时，它直接引起均匀的渗透速度并间接引起非均匀的固体基质剪切应力；因此，稳态渗透协议是探索生长定律的两个相互竞争的假设的良好候选者。分析协议是通过两个 CGFEM 组件的交替交互来实现的：使用 ABAQUS 的多孔弹性有限元分析和使用 MATLAB 的有限元增长例程。 CGFEM 模拟了未成熟牛关节软骨外植体的 12 天生长，其生长规律受到两种相互竞争的假设的影响：一种是由渗透速度触发，另一种是由最大剪切应力触发。结果提供了对生长组织样本的几何、生物力学和生化参数的预测，这些参数可以通过实验测量，因此，建议在进行试点实验时进行分析的关键生物力学措施。 CGFEM 分析和预实验的结合方法可能会完善实际实验方案并更好地理解关节软骨的体外生长。
Recently a cartilage growth finite element model (CGFEM) was developed to solve non-homogeneous and time-dependent growth boundary value problems. The CGFEM allows distinct stress constitutive equations and growth laws for the major components of the solid matrix, collagens and proteoglycans. The objective of the current work was to simulate in vitro growth of articular cartilage explants in a steady-state permeation bioreactor in order to obtain results that aid experimental design. The steady-state permeation protocol induces different types of mechanical stimuli: when the specimen is initially homogeneous it directly induces homogeneous permeation velocities and indirectly induces non-homogeneous solid matrix shear stresses; consequently, the steady-state permeation protocol is a good candidate for exploring two competing hypotheses for the growth laws. The analysis protocols were implemented through the alternating interaction of the two CGFEM components: poroelastic FEA using ABAQUS and a finite element growth routine using MATLAB. The CGFEM simulated 12 days of growth for immature bovine articular cartilage explants subjected to two competing hypotheses for the growth laws: one that is triggered by permeation velocity and the other by maximum shear stress. The results provide predictions for geometric, biomechanical, and biochemical parameters of grown tissue specimens that may be experimentally measured and, consequently, suggest key biomechanical measures to analyze as pilot experiments are performed. The combined approach of CGFEM analysis and pilot experiments may lead to the refinement of actual experimental protocols and a better understanding of in vitro growth of articular cartilage.
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影响因子：
2
作者：
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通讯作者：
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发表时间：
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影响因子：
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通讯作者：
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