Screening of cellular proteins that interact with the classical swine fever virus non-structural protein 5A by yeast two-hybrid analysis
Screening of cellular proteins that interact with the classical swine fever virus non-structural protein 5A by yeast two-hybrid analysis
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通过酵母二杂交分析筛选与猪瘟病毒非结构蛋白5A相互作用的细胞蛋白
DOI：
10.1007/s12038-013-9411-y
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发表时间：
2014-02
影响因子：
2.9
通讯作者：
Zhang, Yanming
中科院分区：
文献类型：
--
作者：
Kang, Kai;Chen, Heng;Xu, Lei;Zhang, Yanming
猪瘟病毒（classical swine fever virus，CSFV）是引起猪瘟（classical swine fever，CSF）的病原，可引起家猪和野猪严重的出血热和血管坏死。大量证据表明，非结构蛋白5A（NS5A）不仅是病毒复制复合体的重要组成部分，而且可以调节宿主细胞的功能，但其作用机制尚不清楚。本研究采用酵母双杂交（Y2H）技术，从猪脐静脉内皮细胞（SUVEC）cDNA文库中筛选与猪瘟病毒NS5A相互作用的蛋白，为深入研究猪瘟病毒NS5A的分子机制提供线索。与NCBI数据库比对发现了16个相互作用蛋白：DDX5、PSMC 3、NAV 1、PHF 5A、GNB 2L1、CSDE1、HSP A8、BRMS 1、PPP2R3C、AIP、TMED 10、POLR1C、TMEM 70、METAP 2、CHORD C1和COPS6。这些蛋白质大多与基因转录、蛋白质折叠、蛋白质降解和代谢有关。Y2H系统检测到的相互作用应被视为初步结果。由于识别在感染过程中发挥重要作用的新途径和宿主靶点，可能为治疗开发提供潜在靶点。从SUVEC cDNA文库中获得的与猪瘟病毒NS5A蛋白相互作用的蛋白质的发现为更好地理解该病毒蛋白与宿主靶蛋白之间的相互作用提供了有价值的信息。
Classical swine fever virus (CSFV), the pathogen of classical swine fever (CSF), causes severe hemorrhagic fever and vascular necrosis in domestic pigs and wild boar. A large number of evidence has proven that non-structural 5A (NS5A) is not only a very important part of viral replication complex, but also can regulate host cell’s function; however, the underlying mechanisms remain poorly understood. In the current study, aiming to find more clues in understanding the molecular mechanisms of CSFV NS5A’s function, the yeast two-hybrid (Y2H) system was adopted to screen for CSFV NS5A interactive proteins in the cDNA library of the swine umbilical vein endothelial cell (SUVEC). Alignment with the NCBI database revealed 16 interactive proteins: DDX5, PSMC3, NAV1, PHF5A, GNB2L1, CSDE1, HSPA8, BRMS1, PPP2R3C, AIP, TMED10, POLR1C, TMEM70, METAP2, CHORDC1 and COPS6. These proteins are mostly related to gene transcription, protein folding, protein degradation and metabolism. The interactions detected by the Y2H system should be considered as preliminary results. Since identifying novel pathways and host targets, which play essential roles during infection, may provide potential targets for therapeutic development. The finding of proteins obtained from the SUVEC cDNA library that interact with the CSFV NS5A protein provide valuable information for better understanding the interactions between this viral protein and the host target proteins.
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DOI：
10.1007/s10541-005-0180-6
发表时间：
2005-07
期刊：
Biochemistry (Moscow)
影响因子：
--
作者：
Wei Sun;Youjia Hu;Jiawei Gong;Chunbao Zhu;Baoquan Zhu
通讯作者：
Wei Sun;Youjia Hu;Jiawei Gong;Chunbao Zhu;Baoquan Zhu
影响因子：
64.5
作者：
Mukouyama, Y;Shin, D;Anderson, DJ
通讯作者：
Anderson, DJ
DOI：
10.1073/pnas.1434429100
发表时间：
2003-09-16
影响因子：
11.1
作者：
Chi, JT;Chang, HY;Brown, PO
通讯作者：
Brown, PO
DOI：
10.1073/pnas.95.26.15183
发表时间：
1998-12-22
影响因子：
11.1
作者：
Griffith, EC;Su, Z;Liu, JO
通讯作者：
Liu, JO
通过酵母双杂交分析与猪瘟病毒非结构蛋白2相关的相互作用细胞蛋白
影响因子：
2.8
作者：
Kang, Kai;Guo, Kangkang;Lin, Zhi
通讯作者：
Lin, Zhi