Synthesis, optical properties and binding interactions of a multivalent glycocluster based on a fluorescent perylene bisimide derivative
Synthesis, optical properties and binding interactions of a multivalent glycocluster based on a fluorescent perylene bisimide derivative
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基于荧光苝双酰亚胺衍生物的多价糖簇的合成、光学性质和结合相互作用
DOI：
10.1039/c3ra44675a
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发表时间：
2013-10
期刊：
影响因子：
3.9
通讯作者：
Xiao-Liu Li*
中科院分区：
文献类型：
--
作者：
Feng Qian,;Yue-Qing Wang;Jin-Chao Zhang;Xiao-Liu Li*
合成了接枝有六个甘露糖基团的水溶性荧光苝双酰亚胺衍生物PBI-Cl-Man。通过紫外-可见光谱和荧光光谱研究其光学性质，表明化合物PBI-Cl-Man在低浓度下以单体状态存在，并表现出高荧光，在水中荧光量子产率为59.9%。此外，通过荧光光谱、CD光谱、浊度测定和ELLA实验研究了PBI-Cl-Man与Con A的结合相互作用。与Con A结合后，观察到荧光几乎完全猝灭，结合常数为2.4 × 107 M−1（单体甘露糖为4.0 × 106 M−1，价态校正），比单价甘露糖配体（α-MMP）高近3个数量级。 ELLA实验表明，PBI-Cl-Man作为多价糖簇，与Con A表现出强烈的结合相互作用，IC50值为34 μM（204 μM，价态校正），比参考化合物（α-MMP）强7倍。
A water-soluble fluorescent perylene bisimide derivative, PBI-Cl-Man, grafted with six mannose groups was synthesized. Its optical properties were investigated by UV-vis and fluorescence spectra, which indicated that compound PBI-Cl-Man exists in the monomer state at low concentrations and exhibits high fluorescence with a fluorescence quantum yield of 59.9% in water. Furthermore, the binding interactions of PBI-Cl-Man with Con A were studied by fluorescence spectra, CD spectra, turbidity assays and ELLA experiments. The almost complete quenching of the fluorescence upon binding with Con A was observed, with a binding constant of 2.4 × 107 M−1 (4.0 × 106 M−1 for monomeric mannose, valency corrected), nearly 3 orders of magnitude higher than that of monovalent mannose ligands (α-MMP). The ELLA experiments indicated that PBI-Cl-Man, as a multivalent glycocluster, exhibits a strong binding interaction with Con A with an IC50 value of 34 μM (204 μM, valency corrected), 7 times stronger than that of the reference compound (α-MMP).
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影响因子：
3.2
作者：
Y. Lim;Myongsoo Lee
通讯作者：
Y. Lim;Myongsoo Lee
影响因子：
3.6
作者：
Chabre, Yoann M.;Brisebois, Patrick P.;Abbassi, Leiela;Kerr, Sheena C.;Fahy, John V.;Marcotte, Isabelle;Roy, Rene
通讯作者：
Roy, Rene
影响因子：
2.7
作者：
Ke-Rang Wang;Yue-qing Wang;Jie Li;Hong-Wei An;Liping Zhang;Jinchao Zhang;Xiao-liu Li
通讯作者：
Ke-Rang Wang;Yue-qing Wang;Jie Li;Hong-Wei An;Liping Zhang;Jinchao Zhang;Xiao-liu Li
DOI：
10.1002/chin.200813271
发表时间：
2007-01
期刊：
ChemInform
影响因子：
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作者：
A. Vargas-Berenguel;F. Ortega-Caballero;J. Casas-Solvas
通讯作者：
A. Vargas-Berenguel;F. Ortega-Caballero;J. Casas-Solvas
影响因子：
4.9
作者：
S. Schmid;A. Mishra;P. Bäuerle
通讯作者：
S. Schmid;A. Mishra;P. Bäuerle