Lactate-dehydrogenase 5 is overexpressed in non-small cell lung cancer and correlates with the expression of the transketolase-like protein 1.
Lactate-dehydrogenase 5 is overexpressed in non-small cell lung cancer and correlates with the expression of the transketolase-like protein 1.
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DOI：
10.1186/1746-1596-5-22
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发表时间：
2010-04-12
影响因子：
2.6
通讯作者：
zur Hausen A
中科院分区：
文献类型：
--
作者：
Kayser G;Kassem A;Sienel W;Schulte-Uentrop L;Mattern D;Aumann K;Stickeler E;Werner M;Passlick B;zur Hausen A
作为五种乳酸脱氢酶（LDH）同工酶之一，LDH5具有最高的催化丙酮酸转化为乳酸的效率。癌细胞中 LDH5 过度表达会诱导糖酵解代谢上调并减少对氧存在的依赖。在这里，我们分析了一大群原发性肺癌中 LDH5 蛋白的表达，并与临床病理数据及其对患者生存的可能影响相关。使用多克隆 LDH5 抗体 (ab53010) 通过免疫组织化学法检测原发性肺癌 (n = 269) 和非肿瘤性肺组织 (n = 35) 的 LDH5 表达。 LDH5表达的结果与临床病理数据以及患者的生存相关。此外，之前测试的转酮醇酶样1蛋白（TKTL1）表达的结果与LDH5表达相关。 89.5% (n = 238) 的 NSCLC 显示 LDH5 表达，而在非肿瘤性肺组织 (n = 34) 中未检测到 LDH5 表达 (p < 0.0001)。 LDH5 过表达与组织学类型相关（腺癌 = 57%，鳞状细胞癌 = 45%，大细胞癌 = 46%，p = 0.006）。在 TNM 分期、分级或生存方面未检测到显着相关性。在整个队列以及每个分级和 pN 组中，可以显示 TKTL1 和 LDH5 表达之间的两侧相关性 (p = 0.002)。无法揭示每种组织学肿瘤类型中 LDH5 和 TKTL1 之间的显着相关性。 LDH5 在 NSCLC 中过度表达，因此可以作为恶性肿瘤的额外标志物。此外，LDH5 与预后标志物 TKTL1 呈正相关。我们的结果证实了这两种代谢酶之间的密切联系，并表明恶性转化过程中葡萄糖代谢的改变。
As one of the five Lactate dehydrogenase (LDH) isoenzymes, LDH5 has the highest efficiency to catalyze pyruvate transformation to lactate. LDH5 overexpression in cancer cells induces an upregulated glycolytic metabolism and reduced dependence on the presence of oxygen. Here we analyzed LDH5 protein expression in a well characterized large cohort of primary lung cancers in correlation to clinico-pathological data and its possible impact on patient survival. Primary lung cancers (n = 269) and non neoplastic lung tissue (n = 35) were tested for LDH5 expression by immunohistochemistry using a polyclonal LDH5 antibody (ab53010). The results of LDH5 expression were correlated to clinico-pathological data as well as to patient's survival. In addition, the results of the previously tested Transketolase like 1 protein (TKTL1) expression were correlated to LDH5 expression. 89.5% (n = 238) of NSCLC revealed LDH5 expression whereas LDH5 expression was not detected in non neoplastic lung tissues (n = 34) (p < 0.0001). LDH5 overexpression was associated with histological type (adenocarcinoma = 57%, squamous cell carcinoma = 45%, large cell carcinoma = 46%, p = 0.006). No significant correlation could be detected with regard to TNM-stage, grading or survival. A two sided correlation between the expression of TKTL1 and LDH5 could be shown (p = 0.002) within the overall cohort as well as for each grading and pN group. A significant correlation between LDH5 and TKTL1 within each histologic tumortype could not be revealed. LDH5 is overexpressed in NSCLC and could hence serve as an additional marker for malignancy. Furthermore, LDH5 correlates positively with the prognostic marker TKTL1. Our results confirm a close link between the two metabolic enzymes and indicate an alteration in the glucose metabolism in the process of malignant transformation.
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影响因子：
2.6
作者：
Schultz H;Kähler D;Branscheid D;Vollmer E;Zabel P;Goldmann T
通讯作者：
Goldmann T
影响因子：
8
作者：
Leiblich, A.;Cross, S. S.;Rehman, I.
通讯作者：
Rehman, I.
非小细胞肺癌组织中乳酸脱氢酶 5 (LDH-5) 过度表达与肿瘤缺氧、血管生成因子产生和不良预后有关。
影响因子：
8.8
作者：
Koukourakis, MI;Giatromanolaki, A;Sivridis, E;Bougioukas, G;Didilis, V;Gatter, KC;Harris, AL
通讯作者：
Harris, AL
影响因子：
6.2
作者：
DORFMAN, HD;CZERNIAK, B
通讯作者：
CZERNIAK, B
影响因子：
2.5
作者：
KAYSER, KW;BULZEBRUCK, H;VOGTMOYKOPF, I
通讯作者：
VOGTMOYKOPF, I