Immunoproteomic to identify antigens in the intestinal mucosa of Crohn's disease patients.
Immunoproteomic to identify antigens in the intestinal mucosa of Crohn's disease patients.
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DOI：
10.1371/journal.pone.0081662
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Ren J
中科院分区：
文献类型：
--
作者：
Zhou Z;Liu H;Gu G;Wang G;Wu W;Zhang C;Ren J
克罗恩病（CD）的发病率在过去十年中显著增加。免疫蛋白质组学是一种很有前途的方法，以确定不同疾病的生物标志物。本研究应用免疫蛋白质组学技术对8例CD患者肠黏膜病变组织及邻近正常肠黏膜组织蛋白质进行了研究。Western blotting检测反应蛋白。在双向电泳凝胶上检测到约50个位于4至7 pI范围内的蛋白质点，并确定了6个10至100 kDa之间的差异表达蛋白质点。反应蛋白被鉴定为抑制素、钙网蛋白、载脂蛋白A-I、intelectin-1、蛋白质二硫键异构酶和谷胱甘肽S-转移酶Pi。对另外4例CD患者的肠粘膜进行Western印迹，以验证反应蛋白。我们发现肠粘膜病变有高水平的抑制素表达。在100%的肠粘膜检查中检测到谷氨酸转移酶表达。因此，我们报告了6种CD自身抗原，包括3种新的和3种以前报道的自身抗原。白细胞介素-1、蛋白质二硫键异构酶和谷胱甘肽-S-转移酶可用作CD发病机制的生物标志物。
Incidences of Crohn disease (CD) have increased significantly in the last decade. Immunoproteomics are a promising method to identify biomarkers of different diseases. In the present study, we used immunoproteomics to study proteins of intestinal mucosal lesions and neighboring normal intestinal mucosa of 8 CD patients. Reactive proteins were validated by Western blotting. Approximately 50 protein spots localized in the 4 to 7 pI range were detected on two-dimensional electrophoresis gels, and 6 differentially expressed protein spots between 10 and 100 kDa were identified. Reactive proteins were identified as prohibitin, calreticulin, apolipoprotein A-I, intelectin-1, protein disulfide isomerase, and glutathione s-transferase Pi. Western blotting was conducted on the intestinal mucosa of another 4 CD patients to validate the reactive proteins. We found that intestinal mucosal lesions had high levels of prohibitin expression. Glutathione s-transferase expression was detected in 100% of the intestinal mucosa examined. Thus, we report 6 autoantigens of CD, including 3 new and 3 previously reported autoantigens. Intelectin-1, protein disulfide isomerase, and glutathione-s-transferases may be used as biomarkers for CD pathogenesis.
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影响因子：
2.3
作者：
Dubinsky, Marla
通讯作者：
Dubinsky, Marla
影响因子：
3.9
作者：
Melmed, Gil Y.;Fleshner, Phillip R.;Targan, Stephan R.
通讯作者：
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作者：
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作者：
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Weber, P
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3.7
作者：
Kathiria AS;Butcher LD;Feagins LA;Souza RF;Boland CR;Theiss AL
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Theiss AL