High resolution ultrasonic neural modulation observed via in vivo two-photon calcium imaging.
High resolution ultrasonic neural modulation observed via in vivo two-photon calcium imaging.
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DOI：
10.1016/j.brs.2021.12.005
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发表时间：
2022-01
影响因子：
7.7
通讯作者：
Cui, Meng
中科院分区：
文献类型：
--
作者：
Cheng, Zongyue;Wang, Chenmao;Wei, Bowen;Gan, Wenbiao;Zhou, Qifa;Cui, Meng
神经调制在描述电路机制方面起着重要作用，并且是神经接口技术的基石。在各种调制机制中，超声能够无创无标记地深入哺乳动物脑组织。到目前为止，大多数（如果不是全部）超声波神经调制实现都基于~1 MHz载波频率。长的声波波长导致空间上粗糙的调制区，通常跨越多个功能区域。一个功能区域的调节不可避免地与其相邻区域的调节相联系。此外，在大多数研究中缺乏体内细胞分辨率细胞类型特异性记录能力，这阻止了对超声的真实细胞反应的揭示。为了显著提高空间分辨率，我们探索了高频超声的应用。为了研究细胞分辨率下的神经元反应，我们开发了一种结合体内双光子钙成像和聚焦超声调制的双模态系统。研究表明，~30 MHz的超声可以抑制清醒小鼠在100 mm尺度空间分辨率下的神经元活动，为高分辨率超声神经调制铺平了道路。通过本研究验证的双模态体内系统将作为研究各种细胞类型响应超声的动力学的通用平台。
Neural modulation plays a major role in delineating the circuit mechanisms and serves as the cornerstone of neural interface technologies. Among the various modulation mechanisms, ultrasound enables noninvasive label-free deep access to mammalian brain tissue. To date, most if not all ultrasonic neural modulation implementations are based on ~1 MHz carrier frequency. The long acoustic wavelength results in a spatially coarse modulation zone, often spanning over multiple function regions. The modulation of one function region is inevitably linked with the modulation of its neighboring regions. Moreover, the lack of in vivo cellular resolution cell-type-specific recording capabilities in most studies prevents the revealing of the genuine cellular response to ultrasound. To significantly increase the spatial resolution, we explored the application of high-frequency ultrasound. To investigate the neuronal response at cellular resolutions, we developed a dual-modality system combining in vivo two-photon calcium imaging and focused ultrasound modulation. The studies show that the ~30 MHz ultrasound can suppress the neuronal activity in awake mice at 100-mm scale spatial resolutions, paving the way for high-resolution ultrasonic neural modulation. The dual-modality in vivo system validated through this study will serve as a general platform for studying the dynamics of various cell types in response to ultrasound.
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影响因子：
16.2
作者：
Guo, Hongsun;Hamilton, Mark, II;Lim, Hubert H.
通讯作者：
Lim, Hubert H.
DOI：
10.1109/tuffc.2006.1588408
发表时间：
2006-01-01
影响因子：
3.6
作者：
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通讯作者：
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作者：
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影响因子：
3.6
作者：
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