Neural correlates of migration: activation of hypothalamic clock(s) in and out of migratory state in the blackheaded bunting (Emberiza melanocephala).
Neural correlates of migration: activation of hypothalamic clock(s) in and out of migratory state in the blackheaded bunting (Emberiza melanocephala).
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DOI：
10.1371/journal.pone.0070065
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Kumar V
中科院分区：
文献类型：
--
作者：
Rastogi A;Kumari Y;Rani S;Kumar V
许多脊椎动物使用视交叉上核(SCN)来区分白天的长短。特别是鸟类，下丘脑的内侧基底核(MBH)被认为与季节性繁殖的时间有关。本研究研究了SCN和MBH对单一长日照的反应，以及MBH在诱导夜间迁徙的黑头雀迁徙表型中的作用。实验1用免疫细胞化学方法检测照射20h后大鼠脊髓核内c-fos和MBH中c-fos、血管活性肠肽(VIP)和神经肽Y(NPY)的含量。长时间光照诱导MBH中c-fos表达显著增强，但在SCN中无明显变化。在MBH内，c-fos-lir细胞在下丘脑下核(IH)和漏斗状核(IN)明显密集，而在下丘脑背内侧(DMH)无明显变化。IH和IN的VIP和NPY标记细胞数也明显增加。DMH仅使VIP标记细胞数显著增加。实验2分别于白天和夜间、长日(白天活动、非迁徙状态)和祖贡如和(夜间活动、迁徙状态)7天后，对白背地鼠的MBH中c-fos、VIP和NPY免疫活性进行检测。在迁移状态下，c-fos-lir细胞仅在DMH区显著增多；VIP-lir细胞在三个MBH区均较密集，提示夜间光敏性增强。非迁徙状态下，只有IN中的NPY-LiR细胞密度较高，可能是由于迁徙前的过度吞噬。在花束中，SCN可能不参与光周期诱导的季节性反应。MBH包含对日长敏感的季节性时钟。血管活性肠肽和神经肽Y是神经内分泌机制的一部分(S)，它们分别参与感知和翻译季节性反应中的光周期信息。
Many vertebrates distinguish between short and long day lengths using suprachiasmatic nuclei (SCN). In birds particular, the mediobasal hypothalamus (MBH) is suggested to be involved in the timing of seasonal reproduction. This study investigated the response of SCN and MBH to a single long day, and the role of MBH in induction of the migratory phenotype in night-migratory blackheaded buntings. Experiment 1 immunocytochemically measured c-fos in the SCN, and c-fos, vasoactive intestinal peptide (VIP) and neuropeptide Y (NPY) in the MBH of buntings exposed to a 20 h light period. Long light period induced significantly stronger c-fos expression, measured as number of c-fos-like immunoreactive (c-fos-lir) cells, in MBH, but not in the SCN. Within the MBH, c-fos-lir cells were significantly denser in the inferior hypothalamic nucleus (IH) and infundibular nucleus (IN), but not in the dorsomedial hypothalamus (DMH). IH and IN also had significantly increased number of VIP and NPY labeled cells. DMH had significantly increased number of VIP labeled cells only. Experiment 2 assayed c-fos, VIP and NPY immunoreactivities in the middle of day and night in the MBH of buntings, after seven long days (day active, non-migratory state) and after seven days of Zugunruhe (night active, migratory state) in long days. In the migratory state, the number of c-fos-lir cells was significantly greater only in DMH; VIP-lir cells were denser in all three MBH regions suggesting enhanced light sensitivity at night. The denser NPY-lir cells only in IN in the non-migratory state were probably due to premigratory hyperphagia. In buntings, SCN may not be involved in the photoperiod-induced seasonal responses. MBH contains the seasonal clock sensitive to day length. VIP and NPY are parts of the neuroendocrine mechanism(s) involved, respectively, in sensing and translating the photoperiodic message in a seasonal response.
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影响因子：
3.5
作者：
Bartell, PA;Gwinner, E
通讯作者：
Gwinner, E
DOI：
10.1152/ajpregu.00805.2004
发表时间：
2005-06-01
影响因子：
2.8
作者：
Kawaguchi, M;Scott, KA;Bi, S
通讯作者：
Bi, S
影响因子：
3
作者：
KUENZEL, WJ;DOUGLASS, LW;DAVISON, BA
通讯作者：
DAVISON, BA
影响因子：
3
作者：
D'Hondt, E;Vermeiren, J;Berghman, LR
通讯作者：
Berghman, LR
影响因子：
2
作者：
KUMAR, V
通讯作者：
KUMAR, V