RNA induces conformational changes in the SF1/U2AF65 splicing factor complex.
RNA induces conformational changes in the SF1/U2AF65 splicing factor complex.
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DOI：
10.1016/j.jmb.2010.11.054
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发表时间：
2011-02-04
影响因子：
5.6
通讯作者：
Kielkopf CL
中科院分区：
文献类型：
--
作者：
Gupta A;Jenkins JL;Kielkopf CL
剪接体通过一系列动态的核糖核蛋白复合体在mRNA前剪接位点上组装，但构象变化的性质尚不清楚。剪接因子1(SF1)和U2辅助因子(U2AF65)在前mRNA剪接的初始阶段协同识别3‘剪接位点。在这里，我们使用小角X射线散射来比较SF1和U2AF65剪接因子的分子尺寸和从头算形状修复，以及在没有和存在AdML剪接位点RNA的情况下SF1/U2AF65复合体的分子尺寸和从头算形状恢复。在没有RNA配体的情况下，SF1/U2AF65/RNA复合体的分子尺寸相对于SF1/U2AF65复合体的最大尺寸基本上收缩了15？相反，分离的SF1和U2AF65剪接因子或它们与RNA的单独复合物没有观察到可检测到的变化，尽管它们的分子被膜形状略有不同。我们认为，由SF1/U2AF65/RNA复合体组装引起的构象变化为随后的剪接阶段定位前mRNA剪接位点提供了最佳的位置。
Spliceosomes assemble on pre-mRNA splice sites through a series of dynamic ribonucleoprotein complexes, yet the nature of the conformational changes remains unclear. Splicing Factor 1 (SF1) and U2 Auxiliary Factor (U2AF65) cooperatively recognize the 3’ splice site during the initial stages of pre-mRNA splicing. Here, we used small-angle X-ray scattering to compare the molecular dimensions and ab initio shape restorations of SF1 and U2AF65 splicing factors, as well as the SF1/U2AF65 complex in the absence and presence of AdML splice site RNAs. The molecular dimensions of the SF1/U2AF65/RNA complex substantially contracted by 15 Å in the maximum dimension, relative to the SF1/U2AF65 complex in the absence of RNA ligand. In contrast, no detectable changes were observed for the isolated SF1 and U2AF65 splicing factors or their individual complexes with RNA, although slight differences in the shapes of their molecular envelopes were apparent. We propose that the conformational changes that are induced by assembly of the SF1/U2AF65/RNA complex serve to position the pre-mRNA splice site optimally for subsequent stages of splicing.
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影响因子：
3
作者：
Mertens, Haydyn D. T.;Svergun, Dmitri I.
通讯作者：
Svergun, Dmitri I.
影响因子：
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作者：
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