Reconstructing Soma-Soma Synapse-like Vesicular Exocytosis with DNA Origami.
Reconstructing Soma-Soma Synapse-like Vesicular Exocytosis with DNA Origami.
复制标题
用 DNA 折纸重建体-体突触样囊泡胞吐作用
DOI：
10.1021/acscentsci.1c00645
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发表时间：
2021-08-25
影响因子：
18.2
通讯作者：
Mao X
中科院分区：
文献类型：
--
作者：
Liu J;Li M;Li F;Ge Z;Li Q;Liu M;Shi J;Wang L;Zuo X;Fan C;Mao X
Cell–cell communications exhibit distinct physiological functions in immune responses and neurotransmitter signaling. Nevertheless, the ability to reconstruct a soma–soma synapse-like junction for probing intercellular communications remains difficult. In this work, we develop a DNA origami nanostructure-based method for establishing cell conjugation, which consequently facilitates the reconstruction of a soma–soma synapse-like junction. We demonstrate that intercellular communications including small molecule and membrane vesicle exchange between cells are maintained in the artificially designed synapse-like junction. By inserting the carbon fiber nanometric electrodes into the soma–soma synapse-like junction, we accomplish the real-time monitoring of individual vesicular exocytotic events and obtain the information on vesicular exocytosis kinetics via analyzing the parameters of current spikes. This strategy provides a versatile platform to study synaptic communications. A soma−soma synapse-like junction was constructed via DNA origami nanostructures. A carbon fiber nanoelectrode was inserted into the junction for vesicular exocytotic monitoring.
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DOI：
10.1002/anie.201504839
发表时间：
2015-10-05
期刊：
Angewandte Chemie (International ed. in English)
影响因子：
--
作者：
Li X;Majdi S;Dunevall J;Fathali H;Ewing AG
通讯作者：
Ewing AG
影响因子：
14.8
作者：
Gartner, Zev J.;Prescher, Jennifer A.;Lavis, Luke D.
通讯作者：
Lavis, Luke D.
DOI：
10.1002/anie.201700095
发表时间：
2017-04-24
期刊：
Angewandte Chemie (International ed. in English)
影响因子：
--
作者：
Ren L;Pour MD;Majdi S;Li X;Malmberg P;Ewing AG
通讯作者：
Ewing AG
实时监测自我重建神经肌肉接头内的离散突触释放事件和兴奋电位（热点论文）
影响因子：
16.6
作者：
Li, Yu-Tao;Zhang, Shu-Hui;Huang, Wei-Hua
通讯作者：
Huang, Wei-Hua
使用工程细胞折纸簇对细胞间通信进行编程
影响因子：
15
作者：
Ge, Zhilei;Liu, Jiangbo;Fan, Chunhai
通讯作者：
Fan, Chunhai