Zinc Regulates Chemical-Transmitter Storage in Nanometer Vesicles and Exocytosis Dynamics as Measured by Amperometry.
Zinc Regulates Chemical-Transmitter Storage in Nanometer Vesicles and Exocytosis Dynamics as Measured by Amperometry.
复制标题
DOI：
10.1002/anie.201700095
复制
发表时间：
2017-04-24
期刊：
影响因子：
--
通讯作者：
Ewing AG
中科院分区：
文献类型：
--
作者：
Ren L;Pour MD;Majdi S;Li X;Malmberg P;Ewing AG
我们应用了纳米尖端电极的电化学技术，表明微摩尔浓度的锌不仅会触发胞吐动力学的变化，还会在模型细胞系中引发囊泡含量的变化。100μM锌处理后，PC12细胞囊泡儿茶酚胺含量显著降低，但胞吐过程中的儿茶酚胺释放基本保持不变。这与胞吐小泡中储存的分子数量形成对比，后者减少，我们发现锌处理细胞释放的儿茶酚胺的量几乎达到100%被排出的含量。进一步的研究表明，锌减缓了胞吐的释放，为这种情况的发生提供了时间。我们的结果提供了锌和神经递质释放过程调节之间缺失的联系，神经递质释放过程可能在记忆形成和存储中起重要作用。我们用单细胞安培法和细胞内囊泡撞击电化学细胞术研究了锌对PC12细胞胞吐和囊泡含量的影响。我们的结果表明，锌不仅改变了递质的储存，而且通过导致更稳定的融合孔开闭过程而改变了胞吐动力学。
We applied electrochemical techniques with nano-tip electrodes to show that micromolar concentrations of zinc not only trigger changes in the dynamics of exocytosis, but also vesicle content in a model cell line. The vesicle catecholamine content in PC12 cells is significantly decreased after 100 μM zinc treatment, but, catecholamine release during exocytosis remains nearly the same. This contrasts the number of molecules stored in the exocytosis vesicles, which decreases, and we find that the amount of catecholamine released from zinc-treated cells reaches nearly 100 percent content being expelled. Further investigation shows that zinc slows down exocytotic release allowing time for this to occur. Our results provide the missing link between zinc and the regulation of neurotransmitter release processes, which might be important in memory formation and storage. We have employed single cell amperometry and intracellular vesicle impact electrochemical cytometry to investigate the effects of zinc on exocytosis and vesicle content in PC12 cells. Our results show that zinc not only changes the transmitter storage, but also the exocytosis dynamics by leading a more stable fusion pore opening and closing process.
登录
查看更多内容
DOI：
10.1002/anie.201504839
发表时间：
2015-10-05
期刊：
Angewandte Chemie (International ed. in English)
影响因子：
--
作者：
Li X;Majdi S;Dunevall J;Fathali H;Ewing AG
通讯作者：
Ewing AG
影响因子：
7.4
作者：
Cui, Hui-Fang;Ye, Jian-Shan;Sheu, Fwu-Shan
通讯作者：
Sheu, Fwu-Shan
影响因子：
56.9
作者：
Fisher, RJ;Pevsner, J;Burgoyne, RD
通讯作者：
Burgoyne, RD
影响因子：
4.2
作者：
Frederickson, CJ;Suh, SW;Thompson, RB
通讯作者：
Thompson, RB
影响因子：
5.3
作者：
Colliver, TL;Pyott, SJ;Ewing, AG
通讯作者：
Ewing, AG