Distinct roles of Bcl-2 and Bcl-Xl in the apoptosis of human bone marrow mesenchymal stem cells during differentiation.
Distinct roles of Bcl-2 and Bcl-Xl in the apoptosis of human bone marrow mesenchymal stem cells during differentiation.
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DOI：
10.1371/journal.pone.0019820
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发表时间：
2011-05-12
期刊：
影响因子：
3.7
通讯作者：
Vallette FM
中科院分区：
文献类型：
--
作者：
Oliver L;Hue E;Rossignol J;Bougras G;Hulin P;Naveilhan P;Heymann D;Lescaudron L;Vallette FM
成体间充质干细胞（MSC）可以在活化和分化之前维持很长一段时间。对于维持这些细胞存活的程序知之甚少。未分化的成人间充质干细胞（hMSCs）没有发生凋亡，在不同的细胞死亡诱导剂。相反，当hMSC处于分化的早期阶段时，相同的诱导剂可以容易地诱导细胞凋亡。未分化细胞的存活以完全相反的方式与Bcl-Xl和Bcl-2的表达相关。Bcl-Xl在未分化和分化的hMSC中以相似的水平表达，而Bcl-2仅在分化的细胞中表达。在未分化的hMSCs中，Bcl-Xl的下调与对凋亡的敏感性增加相关，而Bcl-2的异位表达诱导凋亡。这种凋亡与未分化hMSC中胞质努尔77的存在有关。在hMSC中，Bcl-2的表达取决于细胞分化，并且可以是促凋亡的或抗凋亡的。另一方面，Bcl-Xl在所有条件下都表现出抗凋亡活性。
Adult mesenchymal stem cells (MSCs) can be maintained over extended periods of time before activation and differentiation. Little is known about the programs that sustain the survival of these cells. Undifferentiated adult human MSCs (hMSCs) did not undergo apoptosis in response to different cell death inducers. Conversely, the same inducers can readily induce apoptosis when hMSCs are engaged in the early stages of differentiation. The survival of undifferentiated cells is linked to the expression of Bcl-Xl and Bcl-2 in completely opposite ways. Bcl-Xl is expressed at similar levels in undifferentiated and differentiated hMSCs while Bcl-2 is expressed only in differentiated cells. In undifferentiated hMSCs, the down-regulation of Bcl-Xl is associated with an increased sensitivity to apoptosis while the ectopic expression of Bcl-2 induced apoptosis. This apoptosis is linked to the presence of cytoplasmic Nur 77 in undifferentiated hMSCs. In hMSCs, the expression of Bcl-2 depends on cellular differentiation and can be either pro- or anti-apoptotic. Bcl-Xl, on the other hand, exhibits an anti-apoptotic activity under all conditions.
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DOI：
10.1210/me.2008-0161
发表时间：
2008-12
期刊：
Molecular endocrinology (Baltimore, Md.)
影响因子：
--
作者：
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影响因子：
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DOI：
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发表时间：
2005-06-24
影响因子：
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作者：
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影响因子：
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