Genome sequence analyses of two isolates from the recent Escherichia coli outbreak in Germany reveal the emergence of a new pathotype: Entero-Aggregative-Haemorrhagic Escherichia coli (EAHEC).
Genome sequence analyses of two isolates from the recent Escherichia coli outbreak in Germany reveal the emergence of a new pathotype: Entero-Aggregative-Haemorrhagic Escherichia coli (EAHEC).
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DOI：
10.1007/s00203-011-0725-6
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发表时间：
2011-12
影响因子：
2.8
通讯作者：
Daniel, Rolf
中科院分区：
文献类型：
--
作者：
Brzuszkiewicz, Elzbieta;Thuermer, Andrea;Schuldes, Joerg;Leimbach, Andreas;Liesegang, Heiko;Meyer, Frauke-Dorothee;Boelter, Juergen;Petersen, Heiko;Gottschalk, Gerhard;Daniel, Rolf
确定了来自2011年德国大肠杆菌爆发的两名不同患者的两种大肠杆菌O104:H4菌株的基因组序列。分析的两株菌株分别为大肠杆菌GOS1和GOS2（德国暴发菌株）。两个分离株包括一条约5.31 Mbp的染色体和两个推测的质粒。5217 （GOS1）和5224 （GOS2）预测蛋白编码基因与不同大肠杆菌菌株的比较，多位点序列分型分析显示，分离株与肠聚集性大肠杆菌（EAEC）菌株55989最相似。此外，该暴发菌株的一个推定质粒与EAEC菌株的paa型质粒相似，后者含有聚集粘附菌毛操纵子。第二个假定的质粒含有广谱β-内酰胺酶的基因。这种质粒广泛分布于致病性大肠杆菌菌株中。大肠杆菌GOS1和GOS2基因组与EAEC菌株的显著差异是存在编码志贺毒素的前噬菌体，这是肠出血性大肠杆菌（EHEC）菌株的特征。德国暴发菌株的独特基因组特征组合，包含EAEC和EHEC两种病原型的特征，表明它代表了一种新的肠聚集性出血性大肠杆菌（EAHEC）病原型。本文的在线版本（doi:10.1007/s00203-011-0725-6）包含补充材料，仅供授权用户使用。
The genome sequences of two Escherichia coli O104:H4 strains derived from two different patients of the 2011 German E. coli outbreak were determined. The two analyzed strains were designated E. coli GOS1 and GOS2 (German outbreak strain). Both isolates comprise one chromosome of approximately 5.31 Mbp and two putative plasmids. Comparisons of the 5,217 (GOS1) and 5,224 (GOS2) predicted protein-encoding genes with various E. coli strains, and a multilocus sequence typing analysis revealed that the isolates were most similar to the entero-aggregative E. coli (EAEC) strain 55989. In addition, one of the putative plasmids of the outbreak strain is similar to pAA-type plasmids of EAEC strains, which contain aggregative adhesion fimbrial operons. The second putative plasmid harbors genes for extended-spectrum β-lactamases. This type of plasmid is widely distributed in pathogenic E. coli strains. A significant difference of the E. coli GOS1 and GOS2 genomes to those of EAEC strains is the presence of a prophage encoding the Shiga toxin, which is characteristic for enterohemorrhagic E. coli (EHEC) strains. The unique combination of genomic features of the German outbreak strain, containing characteristics from pathotypes EAEC and EHEC, suggested that it represents a new pathotype Entero-Aggregative-Haemorrhagic E scherichia c oli (EAHEC). The online version of this article (doi:10.1007/s00203-011-0725-6) contains supplementary material, which is available to authorized users.
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影响因子：
3.1
作者：
Boisen, Nadia;Struve, Carsten;Nataro, James P.
通讯作者：
Nataro, James P.
影响因子：
3
作者：
Jain E;Bairoch A;Duvaud S;Phan I;Redaschi N;Suzek BE;Martin MJ;McGarvey P;Gasteiger E
通讯作者：
Gasteiger E
影响因子：
3
作者：
LAW, D;GANGULI, LA;ACHESON, DWK
通讯作者：
ACHESON, DWK
影响因子：
3.7
作者：
Chaudhuri RR;Sebaihia M;Hobman JL;Webber MA;Leyton DL;Goldberg MD;Cunningham AF;Scott-Tucker A;Ferguson PR;Thomas CM;Frankel G;Tang CM;Dudley EG;Roberts IS;Rasko DA;Pallen MJ;Parkhill J;Nataro JP;Thomson NR;Henderson IR
通讯作者：
Henderson IR
影响因子：
3.1
作者：
Bernier, C;Gounon, P;Le Bouguénec, C
通讯作者：
Le Bouguénec, C