Highly conserved gene order and numerous novel repetitive elements in genomic regions linked to wing pattern variation in Heliconius butterflies.
Highly conserved gene order and numerous novel repetitive elements in genomic regions linked to wing pattern variation in Heliconius butterflies.
复制标题
DOI：
10.1186/1471-2164-9-345
复制
发表时间：
2008-07-22
期刊：
影响因子：
4.4
通讯作者：
McMillan WO
中科院分区：
文献类型：
--
作者：
Papa R;Morrison CM;Walters JR;Counterman BA;Chen R;Halder G;Ferguson L;Chamberlain N;Ffrench-Constant R;Kapan DD;Jiggins CD;Reed RD;McMillan WO
超过 20 种近亲蝴蝶的翅膀图案颜色各不相同，Heliconiuserato 为适应性辐射提供了一个有趣的例子。与它的拟态Heliconius melpomene的匹配种族一起，H.erato也代表了苗勒拟态的教科书案例，这是一种在有毒生物之间共享共同警告信号的现象。鉴定控制 H.erato 和 H.melpomene 拟态翅膀模式的特定基因非常有意义。为此，我们对这两个物种进行了比较作图和靶向基因组测序。本文报告了与 Heliconius 颜色图案拟态基因相关的基因组序列的比较分析。对 H.erato 幼崽中的 AFLP 多态性进行评分使我们能够调查整个基因组中大约 362 kb 间隔的基因座。通过这种策略，我们能够识别与两个颜色模式基因紧密连锁的标记：D 和 Cr，然后将其用于筛选 H.erato BAC 文库，以识别用于测序的克隆。尽管预测的开放阅读框数量对于昆虫来说是典型的，但 600 kb BAC 序列的基因密度似乎相对较低。我们重点分析了 D 和 Cr 连接的 H.erato BAC 序列以及 Yb 连接的 H. melpomene BAC 序列。 H.erato（Cr 连接的 BAC）和 H.melpomene（Yb 连接的 BAC）同源区域之间的比较分析揭示了两个物种之间高水平的序列保守性和微同线性。我们发现重复元件分别构成 H.erato 和 H.melpomene 的 BAC 序列的 26% 和 20%。这些重复序列中的大多数似乎都是新颖的，因为它们与任何其他可用的昆虫序列没有显着的相似性。我们还观察到 Heliconius 和 Bombyx mori 之间基因顺序的精细保守的迹象，这表明鳞翅目基因组结构可能在很长的进化时间尺度上得到保守。在这里，我们证明了从遗传连锁图谱进展到 Heliconius 蝴蝶基因组序列数据的易处理性。我们还表明，细尺度基因顺序在远缘相关的 Heliconius 物种之间以及 Heliconius 和 B. mori 之间高度保守。总之，这些发现表明巨鳞翅目中的基因组结构可能非常保守，并表明不同鳞翅目物种中的作图和定位克隆工作可以相互提供信息。
With over 20 parapatric races differing in their warningly colored wing patterns, the butterfly Heliconius erato provides a fascinating example of an adaptive radiation. Together with matching races of its co-mimic Heliconius melpomene, H. erato also represents a textbook case of Müllerian mimicry, a phenomenon where common warning signals are shared amongst noxious organisms. It is of great interest to identify the specific genes that control the mimetic wing patterns of H. erato and H. melpomene. To this end we have undertaken comparative mapping and targeted genomic sequencing in both species. This paper reports on a comparative analysis of genomic sequences linked to color pattern mimicry genes in Heliconius. Scoring AFLP polymorphisms in H. erato broods allowed us to survey loci at approximately 362 kb intervals across the genome. With this strategy we were able to identify markers tightly linked to two color pattern genes: D and Cr, which were then used to screen H. erato BAC libraries in order to identify clones for sequencing. Gene density across 600 kb of BAC sequences appeared relatively low, although the number of predicted open reading frames was typical for an insect. We focused analyses on the D- and Cr-linked H. erato BAC sequences and on the Yb-linked H. melpomene BAC sequence. A comparative analysis between homologous regions of H. erato (Cr-linked BAC) and H. melpomene (Yb-linked BAC) revealed high levels of sequence conservation and microsynteny between the two species. We found that repeated elements constitute 26% and 20% of BAC sequences from H. erato and H. melpomene respectively. The majority of these repetitive sequences appear to be novel, as they showed no significant similarity to any other available insect sequences. We also observed signs of fine scale conservation of gene order between Heliconius and the moth Bombyx mori, suggesting that lepidopteran genome architecture may be conserved over very long evolutionary time scales. Here we have demonstrated the tractability of progressing from a genetic linkage map to genomic sequence data in Heliconius butterflies. We have also shown that fine-scale gene order is highly conserved between distantly related Heliconius species, and also between Heliconius and B. mori. Together, these findings suggest that genome structure in macrolepidoptera might be very conserved, and show that mapping and positional cloning efforts in different lepidopteran species can be reciprocally informative.
登录
查看更多内容
影响因子：
1.7
作者：
Jurka, J;Kapitonov, VV;Walichiewicz, J
通讯作者：
Walichiewicz, J
影响因子：
9.8
作者：
Joron M;Papa R;Beltrán M;Chamberlain N;Mavárez J;Baxter S;Abanto M;Bermingham E;Humphray SJ;Rogers J;Beasley H;Barlow K;ffrench-Constant RH;Mallet J;McMillan WO;Jiggins CD
通讯作者：
Jiggins CD
影响因子：
64.8
作者：
Jiggins, CD;Naisbit, RE;Mallet, J
通讯作者：
Mallet, J
影响因子：
64.8
作者：
Kellis, M;Patterson, N;Lander, ES
通讯作者：
Lander, ES
影响因子：
14.9
作者：
Nickerson, DA;Tobe, VO;Taylor, SL
通讯作者：
Taylor, SL