Validation of operant social motivation paradigms using BTBR T+tf/J and C57BL/6J inbred mouse strains.
Validation of operant social motivation paradigms using BTBR T+tf/J and C57BL/6J inbred mouse strains.
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DOI：
10.1002/brb3.273
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发表时间：
2014-09
影响因子：
3.1
通讯作者：
Wood, Caleb
中科院分区：
文献类型：
--
作者：
Martin, Loren;Sample, Hannah;Gregg, Michael;Wood, Caleb
随着自闭症谱系障碍（ASD）的病因被确定，需要新的检测方法来更好地研究这些因素的动物模型。最近的证据表明，社会动机的缺陷是ASD行为的核心，社会动机的定量测量的发展尤为重要。我们研究的目的是开发和验证新的测定方法来定量测量小鼠的社会动机。为了测试我们的范例的有效性，我们比较了BTBR株，记录的社会赤字，亲社会的C57 BL/6 J株。两种新的条件反射模式被开发出来，允许测试小鼠控制对社会伙伴的访问。在社会动机任务中，实验小鼠按下杠杆以获得社会奖励。奖励应急被设置在一个渐进的比例的强化和杠杆按压次数达到了在最后一次审判的测试会议（断点）被用作社会动机的指数。在效价比较任务中，食物奖励的动机与社会奖励进行了比较。我们还探讨了活动，社会联系，并通过一系列的任务，使用任意迷宫视频跟踪系统在一个开放的领域竞技场的社会新奇的偏好。BTBR小鼠在社会动机范式中的断点显著低于C57 BL/6 J小鼠。然而，效价比较任务显示，BTBR小鼠也明显减少了食物奖励的杠杆按压。条件反射范式的结果表明，BTBR株有动机行为的整体赤字。此外，旷场观察的结果可能表明，BTBR品系的社会差异是焦虑诱导的。
As purported causal factors are identified for autism spectrum disorder (ASD), new assays are needed to better phenotype animal models designed to explore these factors. With recent evidence suggesting that deficits in social motivation are at the core of ASD behavior, the development of quantitative measures of social motivation is particularly important. The goal of our study was to develop and validate novel assays to quantitatively measure social motivation in mice. In order to test the validity of our paradigms, we compared the BTBR strain, with documented social deficits, to the prosocial C57BL/6J strain. Two novel conditioning paradigms were developed that allowed the test mouse to control access to a social partner. In the social motivation task, the test mice lever pressed for a social reward. The reward contingency was set on a progressive ratio of reinforcement and the number of lever presses achieved in the final trial of a testing session (breakpoint) was used as an index of social motivation. In the valence comparison task, motivation for a food reward was compared to a social reward. We also explored activity, social affiliation, and preference for social novelty through a series of tasks using an ANY-Maze video-tracking system in an open-field arena. BTBR mice had significantly lower breakpoints in the social motivation paradigm than C57BL/6J mice. However, the valence comparison task revealed that BTBR mice also made significantly fewer lever presses for a food reward. The results of the conditioning paradigms suggest that the BTBR strain has an overall deficit in motivated behavior. Furthermore, the results of the open-field observations may suggest that social differences in the BTBR strain are anxiety induced.
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影响因子：
2.7
作者：
Pobbe, Roger L. H.;Defensor, Erwin B.;Pearson, Brandon L.;Bolivar, Valerie J.;Blanchard, D. Caroline;Blanchard, Robert J.
通讯作者：
Blanchard, Robert J.
影响因子：
2.5
作者：
McFarlane, H. G.;Kusek, G. K.;Crawley, J. N.
通讯作者：
Crawley, J. N.
DOI：
10.1111/j.1460-9568.2009.07073.x
发表时间：
2010-02
期刊：
The European journal of neuroscience
影响因子：
--
作者：
Martin LA;Goldowitz D;Mittleman G
通讯作者：
Mittleman G
影响因子：
1.9
作者：
Matthews, TJ;Abdelbaky, P;Pfaff, DW
通讯作者：
Pfaff, DW
影响因子：
4.7
作者：
Yang, Mu;Perry, Kayla;Weber, Michael D.;Katz, Adam M.;Crawley, Jacqueline N.
通讯作者：
Crawley, Jacqueline N.