The known immunologically active components of Astragalus account for only a small proportion of the immunological adjuvant activity when combined with conjugate vaccines.
The known immunologically active components of Astragalus account for only a small proportion of the immunological adjuvant activity when combined with conjugate vaccines.
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DOI：
10.1055/s-0030-1250574
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发表时间：
2011-05
期刊：
影响因子：
2.7
通讯作者：
Livingston PO
中科院分区：
文献类型：
--
作者：
Hong F;Xiao W;Ragupathi G;Lau CB;Leung PC;Yeung KS;George C;Cassileth B;Kennelly E;Livingston PO
黄芪的95%乙醇提取物已被证明具有有效的活性，当与各种类型的疫苗一起使用时，可作为免疫佐剂。我们在这里努力确定这种提取物中负责这种辅助活性的成分。将与癌症碳水化合物抗原globo H和GD3和癌症肽抗原MUC1结合的KLH与黄芪不同部位或市售黄芪皂苷和黄酮类化合物联合免疫小鼠。用ELISA法测定对肿瘤抗原和KLH的抗体反应，并通过体重减轻计算毒性。黄芪甲苷和黄芪甲苷活性最高，但毒性差异较大。黄芪甲苷II是黄芪中毒性最大的成分，在500µg剂量下体重减轻5-10%，而黄芪甲苷IV在该剂量下完全没有体重减轻，提示黄芪甲苷IV可能在未来的研究中用作免疫佐剂。几种黄酮类化合物也具有显著的辅助活性。然而，当这些已知的黄芪（和内毒素）的免疫活性成分的活性是根据它们在95%乙醇提取物中存在的程度来计算时，它们只提供了一小部分免疫活性。这就提出了一种可能性，即黄芪的其他独特活性成分可能有助于辅助活性，或者黄芪的辅助活性大于其各部分的活性总和。
The 95% ethanol extract of Astragalus has been demonstrated to have potent activity as an immunological adjuvant when administered with vaccines of various types. We endeavor here to identify the components of this extract that are responsible for this adjuvant activity. Mice were immunized with KLH conjugated to cancer carbohydrate antigens globo H and GD3 and cancer peptide antigen MUC1 combined with different Astragalus fractions or with commercially available Astragalus saponins and flavonoids. The antibody responses against cancer antigens and KLH were quantitated in ELISA assays, and toxicity was calculated by weight loss. Astragalosides II and IV were the most active components, but the toxicity of these two differed dramatically. Astragaloside II was the most toxic Astragalus component with 5–10% weight loss at a dose of 500 µg while astragaloside IV showed no weight loss at all at this dose, suggesting that astragaloside IV might be utilized as an immunological adjuvant in future studies. Several flavonoids also had significant adjuvant activity. However, when the activities of these known immunologically active components of Astragalus (and of endotoxin) are calculated based on the extent of their presence in the 95% ethanol extract, they provide only a small proportion of the immunological activity. This raises the possibility that additional uniquely active components of Astragalus may contribute to adjuvant activity, or that the adjuvant activity of Astragalus is greater than the activity of the sum of its parts.
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影响因子：
5.5
作者：
Yang, ZG;Sun, HX;Fang, WH
通讯作者：
Fang, WH
DOI：
10.1016/s0162-3109(99)00166-6
发表时间：
2000-02-01
期刊：
IMMUNOPHARMACOLOGY
影响因子：
--
作者：
Matsumoto, T;Sakurai, MH;Yamada, H
通讯作者：
Yamada, H
DOI：
10.1016/s0162-3109(99)00013-2
发表时间：
1999-05-01
期刊：
IMMUNOPHARMACOLOGY
影响因子：
--
作者：
Ross, GD;Vetvicka, V;Vetvicková, J
通讯作者：
Vetvicková, J
影响因子：
15
作者：
Kim, Yong-Jae;Wang, Pengfei;Gin, David Y.
通讯作者：
Gin, David Y.
影响因子：
6.1
作者：
Maheshwari, RK;Singh, AK;Srimal, RC
通讯作者：
Srimal, RC