The grapevine (Vitis vinifera) LysM receptor kinases VvLYK1-1 and VvLYK1-2 mediate chitooligosaccharide-triggered immunity.
The grapevine (Vitis vinifera) LysM receptor kinases VvLYK1-1 and VvLYK1-2 mediate chitooligosaccharide-triggered immunity.
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DOI：
10.1111/pbi.13017
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发表时间：
2019-04
影响因子：
13.8
通讯作者：
Poinssot B
中科院分区：
文献类型：
--
作者：
Brulé D;Villano C;Davies LJ;Trdá L;Claverie J;Héloir MC;Chiltz A;Adrian M;Darblade B;Tornero P;Stransfeld L;Boutrot F;Zipfel C;Dry IB;Poinssot B
几丁质是真菌细胞壁的主要成分，是一种众所周知的病原体相关分子模式（PAMP），可引发多种哺乳动物和植物物种的防御反应。在这里，我们表明，两个壳寡糖，甲壳素和壳聚糖，作为PAMPs在葡萄藤（葡萄属葡萄），因为它们引发免疫信号事件，防御基因的表达和抗真菌疾病。为了鉴定其同源受体，对葡萄藤家族的LysM受体激酶（LysM-RKs）进行了注释，并对其基因表达谱进行了表征。系统发育分析清楚地区分了三种葡萄LysM-RKs（VvLYKs），它们与介导几丁质诱导的免疫反应的拟南芥几丁质激发子受体激酶1（AtCERK 1）位于同一进化枝。将几丁质感知受损的拟南芥突变体Atcerk 1用编码VvLYK 1 - 1、VvLYK 1 - 2或VvLYK 1 - 3的这三个推定的正向同源基因转化，以确定它们是否会补充Atcerk 1功能的丧失。我们的研究结果提供了证据表明，VvLYK 1 - 1和VvLYK 1 - 2，而不是VvLYK 1 - 3，通过恢复壳寡糖诱导的MAPK激活和免疫基因表达，在功能上补充了Atcerk 1突变体。此外，Atcerk 1中VvLYK 1 - 1的表达恢复了对非适应葡萄藤白粉病（葡萄白粉菌）的渗透抗性。总的来说，我们的结果表明葡萄藤VvLYK 1 - 1和VvLYK 1 - 2参与了几丁质和壳聚糖触发的免疫，并且VvLYK 1 - 1在对葡萄球菌的基础抗性中起着重要作用。
Chitin, a major component of fungal cell walls, is a well‐known pathogen‐associated molecular pattern (PAMP) that triggers defense responses in several mammal and plant species. Here, we show that two chitooligosaccharides, chitin and chitosan, act as PAMPs in grapevine (Vitis vinifera) as they elicit immune signalling events, defense gene expression and resistance against fungal diseases. To identify their cognate receptors, the grapevine family of LysM receptor kinases (LysM‐RKs) was annotated and their gene expression profiles were characterized. Phylogenetic analysis clearly distinguished three V. vinifera LysM‐RKs (VvLYKs) located in the same clade as the Arabidopsis CHITIN ELICITOR RECEPTOR KINASE1 (AtCERK1), which mediates chitin‐induced immune responses. The Arabidopsis mutant Atcerk1, impaired in chitin perception, was transformed with these three putative orthologous genes encoding VvLYK1‐1, ‐2, or ‐3 to determine if they would complement the loss of AtCERK1 function. Our results provide evidence that VvLYK1‐1 and VvLYK1‐2, but not VvLYK1‐3, functionally complement the Atcerk1 mutant by restoring chitooligosaccharide‐induced MAPK activation and immune gene expression. Moreover, expression of VvLYK1‐1 in Atcerk1 restored penetration resistance to the non‐adapted grapevine powdery mildew (Erysiphe necator). On the whole, our results indicate that the grapevine VvLYK1‐1 and VvLYK1‐2 participate in chitin‐ and chitosan‐triggered immunity and that VvLYK1‐1 plays an important role in basal resistance against E. necator.
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影响因子：
3.7
作者：
Gauthier A;Trouvelot S;Kelloniemi J;Frettinger P;Wendehenne D;Daire X;Joubert JM;Ferrarini A;Delledonne M;Flors V;Poinssot B
通讯作者：
Poinssot B
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64.8
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7.2
作者：
Clough, SJ;Bent, AF
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