Synaptic connections between endomorphin 2-immunoreactive terminals and μ-opioid receptor-expressing neurons in the sacral parasympathetic nucleus of the rat.
Synaptic connections between endomorphin 2-immunoreactive terminals and μ-opioid receptor-expressing neurons in the sacral parasympathetic nucleus of the rat.
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大鼠骶副交感核中内吗啡肽 2 免疫反应末端与表达 mu-阿片受体的神经元之间的突触连接
DOI：
10.1371/journal.pone.0062028
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Li YQ
中科院分区：
文献类型：
--
作者：
Dou XL;Qin RL;Qu J;Liao YH;Lu Yc;Zhang T;Shao C;Li YQ
The urinary bladder is innervated by parasympathetic preganglionic neurons (PPNs) that express μ-opioid receptors (MOR) in the sacral parasympathetic nucleus (SPN) at lumbosacral segments L6-S1. The SPN also contains endomorphin 2 (EM2)-immunoreactive (IR) fibers and terminals. EM2 is the endogenous ligand of MOR. In the present study, retrograde tract-tracing with cholera toxin subunit b (CTb) or wheat germ agglutinin-conjugated horseradish peroxidase (WGA-HRP) via the pelvic nerve combined with immunohistochemical staining for EM2 and MOR to identify PPNs within the SPN as well as synaptic connections between the EM2-IR terminals and MOR-expressing PPNs in the SPN of the rat. After CTb was injected into the pelvic nerve, CTb retrogradely labeled neurons were almost exclusively located in the lateral part of the intermediolateral gray matter at L6-S1 of the lumbosacral spinal cord. All of the them also expressed MOR. EM2-IR terminals formed symmetric synapses with MOR-IR, WGA-HRP-labeled and WGA-HRP/MOR double-labeled neuronal cell bodies and dendrites within the SPN. These results provided morphological evidence that EM2-containing axon terminals formed symmetric synapses with MOR-expressing PPNs in the SPN. The present results also show that EM2 and MOR might be involved in both the homeostatic control and information transmission of micturition.
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DOI：
10.1254/jjp.48.31
发表时间：
1988-09-01
期刊：
JAPANESE JOURNAL OF PHARMACOLOGY
影响因子：
--
作者：
KONTANI, H;KAWABATA, Y
通讯作者：
KAWABATA, Y
影响因子：
--
作者：
Blok, BFM
通讯作者：
Blok, BFM
影响因子：
3.3
作者：
GOUARDERES, C;BEAUDET, A;QUIRION, R
通讯作者：
QUIRION, R
影响因子：
2.9
作者：
HISAMITSU, T;DEGROAT, WC
通讯作者：
DEGROAT, WC
影响因子：
3.3
作者：
Banrezes, B;Andrey, P;Maurin, Y
通讯作者：
Maurin, Y