Image-based adaptive optics for two-photon microscopy.
Image-based adaptive optics for two-photon microscopy.
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DOI:
10.1364/ol.34.002495
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发表时间:
2009-08-15
期刊:
影响因子:
3.6
通讯作者:
Wilson T
中科院分区:
文献类型:
--
作者:
Débarre D;Botcherby EJ;Watanabe T;Srinivas S;Booth MJ;Wilson T
We demonstrate wavefront sensorless aberration correction in a two-photon excited fluorescence microscope. Using analysis of the image-formation process, we have developed an optimized correction scheme permitting image-quality improvement with minimal additional exposure of the sample. We show that, as a result, our correction process induces little photobleaching and significantly improves the quality of images of biological samples. In particular, increased visibility of small structures is demonstrated. Finally, we illustrate the use of this technique on various fresh and fixed biological tissues.
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影响因子:
1.9
作者:
Booth, M;Wilson, T;Kawata, S
通讯作者:
Kawata, S
影响因子:
3.8
作者:
Debarre, Delphine;Botcherby, Edward J.;Wilson, Tony
通讯作者:
Wilson, Tony
DOI:
10.1364/josaa.17.001098
发表时间:
2000-06-01
影响因子:
1.9
作者:
Neil, MAA;Booth, MJ;Wilson, T
通讯作者:
Wilson, T
DOI:
10.1073/pnas.082544799
发表时间:
2002-04-30
影响因子:
11.1
作者:
Booth, MJ;Neil, MAA;Wilson, T
通讯作者:
Wilson, T
影响因子:
3.8
作者:
Marsh, PN;Burns, D;Girkin, JM
通讯作者:
Girkin, JM