Image-based adaptive optics for two-photon microscopy.

Image-based adaptive optics for two-photon microscopy.
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DOI:
10.1364/ol.34.002495
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发表时间:
2009-08-15
期刊:
影响因子:
3.6
通讯作者:
Wilson T
Wilson T
中科院分区:
物理与天体物理2区
文献类型:
--
作者:
Débarre D;Botcherby EJ;Watanabe T;Srinivas S;Booth MJ;Wilson T

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我们在双光子激发荧光显微镜中演示了无波前传感器像差校正。使用图像形成过程的分析,我们已经开发出一种优化的校正方案,允许图像质量的改善与最小的额外曝光的样品。我们表明,作为一个结果,我们的校正过程引起的光漂白很少,并显着提高了生物样品的图像质量。特别是,小结构的可见性增加被证明。最后,我们说明了使用这种技术对各种新鲜和固定的生物组织。
We demonstrate wavefront sensorless aberration correction in a two-photon excited fluorescence microscope. Using analysis of the image-formation process, we have developed an optimized correction scheme permitting image-quality improvement with minimal additional exposure of the sample. We show that, as a result, our correction process induces little photobleaching and significantly improves the quality of images of biological samples. In particular, increased visibility of small structures is demonstrated. Finally, we illustrate the use of this technique on various fresh and fixed biological tissues.
DOI: 10.1364/ao.44.005131
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期刊: APPLIED OPTICS
影响因子: 1.9
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