Trans-4-hydroxy-L-proline production by the cyanobacterium Synechocystis sp. PCC 6803.
Trans-4-hydroxy-L-proline production by the cyanobacterium Synechocystis sp. PCC 6803.
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聚囊蓝细菌pcc6803生产反式4-羟基- l -脯氨酸的研究。
DOI：
10.1016/j.mec.2020.e00155
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发表时间：
2021-06
影响因子：
5.2
通讯作者：
Krömer JO
中科院分区：
文献类型：
--
作者：
Brandenburg F;Theodosiou E;Bertelmann C;Grund M;Klähn S;Schmid A;Krömer JO
蓝藻在光生物技术中扮演着重要的角色。然而，它们的关键中心代谢途径之一，三羧酸(TCA)循环，与大多数异养生物相比，具有独特的结构，并且在很大程度上仍未被开发。2-氧基戊二酸通过琥珀酰辅酶A转化为琥珀酸酯是不存在的，但被其他几个反应绕过了。总体而言，光自养生长条件下通过TCA循环的通量很低，这对化学品的生产有影响。在这项研究中，我们研究了聚球藻PCC6803的TCA循环生产反式-4-羟基-L-脯氨酸(Hyp)的能力，反式-4-羟基-L-Pro是制药和化妆品工业中有价值的手性构建块。首次在表达L-脯氨酸-4-羟基酶(P4H)基因的蓝藻中实现了光自养Hyp的产生。RH1菌株。有趣的是，虽然在所有测试条件下都能在重组聚球藻中检测到较高的胞内Hyp浓度，但只有当培养基的pH值超过9.5时才能观察到Hyp分泌到培养基中，而且主要是在培养的后期。我们比较了自养Hyp在大肠杆菌中的生产速率与已公布的基于糖的生产速率。光营养过程的土地利用效率(空间-时间产量)已经与考虑糖料种植的异养过程处于相同的数量级。但是，蓝藻TCA循环的显著可塑性保证了当使用聚球藻时时空产量增加23-55倍的潜力。总之，这些发现有助于更好地理解光自养生物中TCA循环的生物生产，并拓宽代谢工程蓝藻产生的化学物质的光谱。反式-4-羟基-L-脯氨酸的光营养生产。聚球藻PCC6803产物积累的pH依赖性光养和异养植物土地利用效率的比较分析。
Cyanobacteria play an important role in photobiotechnology. Yet, one of their key central metabolic pathways, the tricarboxylic acid (TCA) cycle, has a unique architecture compared to most heterotrophs and still remains largely unexploited. The conversion of 2-oxoglutarate to succinate via succinyl-CoA is absent but is by-passed by several other reactions. Overall, fluxes under photoautotrophic growth conditions through the TCA cycle are low, which has implications for the production of chemicals. In this study, we investigate the capacity of the TCA cycle of Synechocystis sp PCC 6803 for the production of trans-4-hydroxy-L-proline (Hyp), a valuable chiral building block for the pharmaceutical and cosmetic industries. For the first time, photoautotrophic Hyp production was achieved in a cyanobacterium expressing the gene for the L-proline-4-hydroxylase (P4H) from Dactylosporangium sp. strain RH1. Interestingly, while elevated intracellular Hyp concentrations could be detected in the recombinant Synechocystis strains under all tested conditions, detectable Hyp secretion into the medium was only observed when the pH of the medium exceeded 9.5 and mostly in the late phases of the cultivation. We compared the rates obtained for autotrophic Hyp production with published sugar-based production rates in E. coli. The land-use efficiency (space-time yield) of the phototrophic process is already in the same order of magnitude as the heterotrophic process considering sugar farming as well. But, the remarkable plasticity of the cyanobacterial TCA cycle promises the potential for a 23–55 fold increase in space-time yield when using Synechocystis. Altogether, these findings contribute to a better understanding of bioproduction from the TCA cycle in photoautotrophs and broaden the spectrum of chemicals produced in metabolically engineered cyanobacteria. Phototrophic production of trans-4-hydroxy-L-prolin. pH dependency of product accumulation in Synechocystis PCC6803. Comparative analysis of land use efficiency in phototrophs & heterotrophs.
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影响因子：
2.9
作者：
GOOD, NE;WINGET, GD;SINGH, RMM
通讯作者：
SINGH, RMM
DOI：
10.1016/j.jrras.2014.03.002
发表时间：
2014-04-01
影响因子：
1.7
作者：
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7.4
作者：
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通讯作者：
Hagemann, Martin
影响因子：
4.7
作者：
Hoschek, Anna;Toepel, Joerg;Schmid, Andreas
通讯作者：
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影响因子：
3.8
作者：
David, Christian;Schmid, Andreas;Buehler, Katja
通讯作者：
Buehler, Katja