Profiling of differentially expressed proteins between fresh and frozen-thawed Duroc boar semen using ProteinChip CM10.
Profiling of differentially expressed proteins between fresh and frozen-thawed Duroc boar semen using ProteinChip CM10.
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DOI：
10.5187/jast.2023.e27
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发表时间：
2023-03
影响因子：
2.3
通讯作者：
Chung HJ
中科院分区：
文献类型：
--
作者：
Kim YM;Park SW;Lee MJ;Jeon DY;Sa SJ;Jeong YD;Seong HS;Choi JW;Hochi S;Cho ES;Chung HJ
已经进行了许多研究来改进猪精液冷冻保存技术。然而，精子活力和形态的计算机辅助分析不足以预测冻融精液的分子功能。使用同量异序标签进行相对和绝对定量 (iTRAQ) 技术可以得出公猪精子蛋白更准确的表达模式。在本研究中，iTRAQ 标记系统与液相色谱串联质谱 (LC-MS/MS) 分析相结合，以鉴定新鲜和冻融公猪精液之间差异表达的 CM10 分级蛋白。总共鉴定出76种蛋白质类型存在差异表达，其中分别有9种和67种蛋白质在冻融精子样本中的表达量高于新鲜精子样本中的表达量。这些蛋白质的分类功能包括氧化磷酸化、线粒体内膜和基质以及参与三磷酸腺苷（ATP）合成的丙酮酸代谢过程；以及精子鞭毛和活动纤毛，它们参与精子尾部结构。这些结果表明可能存在与杜洛克公猪精液冷冻保存后存活相关的生物标志物网络。
Many studies have been conducted to improve technology for semen cryopreservation in pigs. However, computer-assisted analysis of sperm motility and morphology is insufficient to predict the molecular function of frozen-thawed semen. More accurate expression patterns of boar sperm proteins may be derived using the isobaric tags for relative and absolute quantification (iTRAQ) technique. In this study, the iTRAQ-labeling system was coupled with liquid chromatography tandem-mass spectrometry (LC-MS/MS) analysis to identify differentially expressed CM10-fractionated proteins between fresh and frozen-thawed boar semen. A total of 76 protein types were identified to be differentially expressed, among which 9 and 67 proteins showed higher and lower expression in frozen-thawed than in fresh sperm samples, respectively. The classified functions of these proteins included oxidative phosphorylation, mitochondrial inner membrane and matrix, and pyruvate metabolic processes, which are involved in adenosine triphosphate (ATP) synthesis; and sperm flagellum and motile cilium, which are involved in sperm tail structure. These results suggest a possible network of biomarkers associated with survival after the cryopreservation of Duroc boar semen.
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影响因子：
3.8
作者：
Chen, Xiaoli;Zhu, Huabin;Wang, Dong
通讯作者：
Wang, Dong
DOI：
10.1007/978-1-4939-2193-5_10
发表时间：
2015-01-01
期刊：
CRYOPRESERVATION AND FREEZE- DRYING PROTOCOLS, 3RD EDITION
影响因子：
--
作者：
Sieme, Harald;Oldenhof, Harriette
通讯作者：
Oldenhof, Harriette
影响因子：
3.4
作者：
Wang, Shangqian;Wang, Wei;Yin, Changjun
通讯作者：
Yin, Changjun
影响因子：
2.8
作者：
Vadnais, Melissa L.;Althouse, Gary C.
通讯作者：
Althouse, Gary C.
影响因子：
3.6
作者：
Zilli, L;Schiavone, R;Vilella, S
通讯作者：
Vilella, S