Microwave treatment of the cornea leads to localised disruption of the extracellular matrix.
Microwave treatment of the cornea leads to localised disruption of the extracellular matrix.
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DOI：
10.1038/s41598-018-32110-0
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发表时间：
2018-09-13
影响因子：
4.6
通讯作者：
Quantock AJ
中科院分区：
文献类型：
--
作者：
Morgan SR;Hieda O;Nakai Y;Boote C;Hayes S;Kinoshita S;Meek KM;Quantock AJ
微波角膜移植术是一种热屈光手术，通过微波能量在角膜环周围引起局部收缩，使其变平和视力矫正，可以矫正近视和病理性角膜变陡。然而，对角膜细胞外基质的影响尚未得到评估，因此目前的研究是在兔体内模型中评估手术后超微结构的变化。为了实现这一目标，我们以0.25 mm的间隔对处理过和未处理过的兔角膜进行了一系列小角度x射线散射（SAXS）实验，结果表明胶原纤维内参数（即胶原纤维直径或轴向d周期）没有显著变化，而在微波处理过的角膜区域，纤维间测量（即纤维间距和空间有序程度）明显改变。微波处理角膜的这些结构基质改变预测了角膜生物力学强度和组织透明度的影响，并且，我们认为，潜在地使微波处理的角膜抵抗使用角膜交联程序的手术稳定，目前用于治疗角膜变陡引起的屈光不正。
Microwave keratoplasty is a thermo-refractive surgical procedure that can correct myopia (short-sightedness) and pathologic corneal steepening by using microwave energy to cause localised shrinkage around an annulus of the cornea leading to its flattening and vision correction. The effects on the corneal extracellular matrix, however, have not yet been evaluated, thus the current study to assess post-procedure ultrastructural changes in an in-vivo rabbit model. To achieve this a series of small-angle x-ray scattering (SAXS) experiments were carried out across whole transects of treated and untreated rabbit corneas at 0.25 mm intervals, which indicated no significant change in collagen intra-fibrillar parameters (i.e. collagen fibril diameter or axial D-period), whereas inter-fibrillar measures (i.e. fibril spacing and the degree of spatial order) were markedly altered in microwave-treated regions of the cornea. These structural matrix alterations in microwave-treated corneas have predicted implications for corneal biomechanical strength and tissue transparency, and, we contend, potentially render microwave-treated corneas resistant to surgical stabilization using corneal cross-linking procedures currently employed to combat refractive error caused by corneal steepening.
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