Zonal chondrocytes seeded in a layered agarose hydrogel create engineered cartilage with depth-dependent cellular and mechanical inhomogeneity.
Zonal chondrocytes seeded in a layered agarose hydrogel create engineered cartilage with depth-dependent cellular and mechanical inhomogeneity.
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DOI：
10.1089/ten.tea.2008.0391
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发表时间：
2009-09
影响因子：
--
通讯作者：
Hung CT
中科院分区：
文献类型：
--
作者：
Ng KW;Ateshian GA;Hung CT
假设接种到具有最初规定的深度变化的压缩材料性质的双层支架中的软骨细胞的带状群体将导致具有与天然组织类似的深度依赖性细胞和压缩机械不均匀性的仿生软骨组织构造。分离浅区软骨细胞（SZC）和中/深区软骨细胞（MDZC）并用2%或3%琼脂糖包封以形成2%SZC、3%SZC、2%MDZC的单层构建体; 2%SZC/2%MDZC和3%SZC/2%MDZC的双层构建体;和2%混合软骨细胞对照。对于第42天的SZC，随着琼脂糖浓度的增加，以及当与MDZC分层时，发现GAG和胶原蛋白增加。在双层结构中，表浅区蛋白随着琼脂糖浓度的增加而增加。对于MDZC，当与SZC分层时，观察到GAG含量增加和细胞增殖调节。双层结构具有深度依赖性的压缩模量，这在性质上类似于天然关节软骨，而对照组显示出U形轮廓，外周边缘较硬，中间区域较软。这项研究是第一个创建一个工程软骨组织与深度变化的细胞和机械不均匀性。未来的研究将确定复制不均匀性是否有利于组织工程软骨的临床应用。
It was hypothesized that zonal populations of chondrocytes seeded into a bilayered scaffold with initially prescribed depth-varying, compressive material properties will lead to a biomimetic cartilage tissue construct with depth-dependent cellular and compressive mechanical inhomogeneity similar to the native tissue. Superficial zone chondrocytes (SZCs) and middle/deep zone chondrocytes (MDZCs) were isolated and encapsulated with 2% or 3% agarose to form single layered constructs of 2% SZC, 3% SZC, 2% MDZC; bilayered constructs of 2% SZC / 2% MDZC and 3% SZC / 2% MDZC; and 2% mixed chondrocyte controls. For SZCs on day 42, increased GAG and collagen was found with increased agarose concentration, and when layering with MDZCs. Superficial zone protein increased with increasing agarose concentration in bilayered constructs. For MDZCs, increased GAG content and regulation of cell proliferation was observed when layering with SZCs. Bilayered constructs possessed a depth-dependent compressive modulus that was qualitatively similar to native articular cartilage whereas controls showed a U-shaped profile with stiffer peripheral edges and softer middle region. This study is the first to create an engineered cartilage tissue with both depth-varying cellular and mechanical inhomogeneity. Future studies will determine if replicating inhomogeneity is advantageous in clinical uses of tissue engineered cartilage.
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影响因子：
3.2
作者：
Lin, WQ;Shuster, S;Stern, R
通讯作者：
Stern, R
影响因子：
2.8
作者：
Krishnan, R;Kopacz, M;Ateshian, GA
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Ateshian, GA
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2.9
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影响因子：
--
作者：
Ingber, Donald E.;Mow, Van C.;Vunjak-Novakovic, Gordana
通讯作者：
Vunjak-Novakovic, Gordana