Ubi1 intron-mediated enhancement of the expression of Bt cry1Ah gene in transgenic maize (Zea mays L.)
Ubi1 intron-mediated enhancement of the expression of Bt cry1Ah gene in transgenic maize (Zea mays L.)
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Ubi1 内含子介导的转基因玉米 (Zea mays L.) 中 Btcry1Ah 基因表达的增强
DOI：
10.1007/s11434-008-0379-1
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发表时间：
2008-10
影响因子：
--
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ry1Ah基因是从苏云金芽孢杆菌BT8中克隆的新型杀虫基因之一。构建了两个含有cry1Ah基因的植物表达载体。玉米泛素1基因的第一个内含子插入植物表达载体(pUUOAH)中的玉米泛素启动子和cry1Ah基因之间。通过微弹轰击将这两个载体导入玉米未成熟胚愈伤组织中，获得繁殖植株。 PCR和Southern印迹分析表明，外源基因已整合到玉米基因组中并稳定遗传至下一代。对T1和T2代植物的ELISA检测表明，含有ubi1内含子的构建体(pUUOAH)中Cry1Ah蛋白的表达量比无内含子构建体(pUOAH)高20%。生物测定结果表明，携带cry1Ah基因的转基因玉米对亚洲玉米螟具有较高的抗性，且含有ubi1内含子的转基因玉米的杀虫活性高于无内含子的转基因玉米。这些结果表明玉米ubi1内含子可以有效增强转基因玉米中Btcry1Ah基因的表达
The cry1Ah gene was one of novel insecticidal genes cloned from Bacillus thuringiensis isolate BT8. Two plant expression vectors containing cry1Ah gene were constructed. The first intron of maize ubiqutin1 gene was inserted between the maize Ubiquitin promoter and cry1Ah gene in one of the plant expressing vectors (pUUOAH). The two vectors were introduced into maize immature embryonic calli by microprojectile bombardment, and the reproductively plants were acquired. PCR and Southern blot analysis showed that foreign genes had been integrated into maize genome and inherited to the next generation stably. The ELISA assay to T1 and T2 generation plants showed that the expression of Cry1Ah protein in the construct containing the ubi1 intron (pUUOAH) was 20% higher than that of the intronless construct (pUOAH). Bioassay results showed that the transgenic maize harboring cry1Ah gene had high resistance to the Asian corn borers and the insecticidal activity of the transgenic maize containing the ubi1 intron was higher than that of the intronless construct. These results indicated that the maize ubi1 intron can enhance the expression of the Bt cry1Ah gene in transgenic maize efficiently
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DOI：
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发表时间：
2006
期刊：
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影响因子：
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作者：
C. James
通讯作者：
C. James
影响因子：
10.5
作者：
CALLIS, J;FROMM, M;WALBOT, V
通讯作者：
WALBOT, V
DOI：
--
发表时间：
2001-01
期刊：
--
影响因子：
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作者：
J. Sambrook;E. Fritsch;T. Maniatis
通讯作者：
J. Sambrook;E. Fritsch;T. Maniatis
DOI：
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发表时间：
1990
期刊：
--
影响因子：
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作者：
J. Doyle
通讯作者：
J. Doyle
影响因子：
46.9
作者：
Bates, SL;Zhao, JZ;Shelton, AM
通讯作者：
Shelton, AM