Characterization of a New Transposon, Tn6696, on a bla (NDM-) (1)-Carrying Plasmid From Multidrug-Resistant Enterobacter cloacae ssp. dissolvens in China.
Characterization of a New Transposon, Tn6696, on a bla (NDM-) (1)-Carrying Plasmid From Multidrug-Resistant Enterobacter cloacae ssp. dissolvens in China.
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DOI：
10.3389/fmicb.2020.525479
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发表时间：
2020
影响因子：
5.2
通讯作者：
Song H
中科院分区：
文献类型：
--
作者：
Chen Q;Lin Y;Li Z;Lu L;Li P;Wang K;Yang L;Ma H;Li P;Song H
阴沟肠杆菌是一种条件致病菌，是引起严重医院感染的主要病原菌。在碳青霉烯类耐药中起重要作用的基因是新德里金属-β-内酰胺酶1（NDM-1）。目前，产NDM-1的E.霍乱菌株是一个严重的公共威胁。多药耐药E. Clobacillus ssp.溶出菌株CBG 15936于2017年在中国广州回收。采用PCR、S1脉冲场凝胶电泳和Southern印迹法对blaNDM-1基因进行定位。还进行了药敏试验和偶联实验。使用Illumina HiSeq和Nanopore测序仪进行全基因组测序。菌株CBG 15936属于ST 932，对碳青霉烯类耐药。blaNDM-1基因存在于一个约62-kb的质粒上，其接合频率为1.68 × 10-3个事件/供体细胞。基因组测序和分析表明，携带NDM-1的IncN 1质粒含有一个新的转座子Tn 6696，它由一个完整的qnrS 1携带Tn 6292元件，一个反向的8.3 kb Tn 3000残基，ISkpn 19，ΔtnpA和IS 26组成。在耐药大肠杆菌的blaNDM-1质粒上发现了一个新的转座子Tn 6696。Clobacillus ssp. CBG 15936的溶出度。这一发现为blaNDM-1在耐药菌之间进行水平转移的潜力提供了新的视角。
Enterobacter cloacae is an opportunistic pathogen which is responsible for serious nosocomial infections. A gene which plays an important role in resistance to carbapenems is the New Delhi metallo-β-lactamase 1 (NDM-1). Currently, the spread of NDM-1-producing E. cloacae strains is a serious public threat. A multidrug-resistant E. cloacae ssp. dissolvens strain CBG15936 was recovered in 2017 in Guangzhou, China. PCR, S1-pulsed-field gel electrophoresis, and Southern blotting were performed to locate the blaNDM–1 gene. Susceptibility testing and conjugation experiments were also performed. Illumina HiSeq and Nanopore sequencers were used to perform whole-genome sequencing. Strain CBG15936 belongs to ST932 and is resistant to carbapenems. The blaNDM–1 gene was found on a ∼62-kb plasmid, which has a conjugation frequency of 1.68 × 10–3 events per donor cell. Genome sequencing and analysis revealed that the NDM-1-carrying IncN1 plasmid contained a new transposon Tn6696, which consists of an intact qnrS1-carrying Tn6292 element, an inverted 8.3-kb Tn3000 remnant, ISkpn19, ΔtnpA, and IS26. A new transposon, Tn6696, has been detected on a blaNDM–1-carrying plasmid recovered from multidrug-resistant E. cloacae ssp. dissolvens CBG15936 from China. This finding provides a new perspective regarding the potential for blaNDM–1 to undergo horizontal transfer among drug-resistant bacteria.
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