Natural Attenuation in Streambed Sediment Receiving Chlorinated Solvents from Underlying Fracture Networks.
Natural Attenuation in Streambed Sediment Receiving Chlorinated Solvents from Underlying Fracture Networks.
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DOI：
10.1021/acs.est.6b05554
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发表时间：
2017-05-02
影响因子：
11.4
通讯作者：
Löffler FE
中科院分区：
文献类型：
--
作者：
Şimşir B;Yan J;Im J;Graves D;Löffler FE
断裂基岩地层的污染物排放仍然是修复挑战。我们采用综合方法来评估沉积物的自然衰减潜力，该沉积物形成了来自受氯化溶剂污染的裂隙基岩含水层的上涌地下水与接收地表水之间的过渡区。原位测量表明，沉积物中的还原脱氯在到达水柱之前减弱了氯化化合物。用溪流沉积物建立的微观世界或原位孵育的 Bio-Sep 珠将 C1-C3 氯化溶剂降解为氯化程度较低或无害的产品。定量 PCR 和 16S rRNA 基因扩增子测序揭示了小溪沉积物中已知脱氯剂生物标志物基因的丰度和空间分布，并证明降解氯化 C1-C3 烷烃和烯烃的多个脱氯剂群体共同栖息在沉积物中。细菌和古菌序列的系统发育分类表明在空间（水平300 m）尺度上分布相对均匀，但Dehalococcoides和Dehalobacter在较深层沉积物中更为丰富，每克沉积物分别测量到5.7 ± 0.4 × 105和5.4 ± 0.9 × 106 16S rRNA基因拷贝。微生物学和水文地质特征表明，裂隙基岩-沉积物界面的微生物过程对于防止污染物到达水体至关重要，强调了这一关键区域环境与污染物衰减的相关性。
Contaminant discharge from fractured bedrock formations remains a remediation challenge. We applied an integrated approach to assess the natural attenuation potential of sediment that forms the transition zone between upwelling groundwater from a chlorinated solvent-contaminated fractured bedrock aquifer and the receiving surface water. In situ measurements demonstrated that reductive dechlorination in the sediment attenuated chlorinated compounds before reaching the water column. Microcosms established with creek sediment or in situ incubated Bio-Sep beads degraded C1–C3 chlorinated solvents to less-chlorinated or innocuous products. Quantitative PCR and 16S rRNA gene amplicon sequencing revealed the abundance and spatial distribution of known dechlorinator biomarker genes within the creek sediment and demonstrated that multiple dechlorinator populations degrading chlorinated C1–C3 alkanes and alkenes co-inhabit the sediment. Phylogenetic classification of bacterial and archaeal sequences indicated a relatively uniform distribution over spatial (300 m horizontally) scale, but Dehalococcoides and Dehalobacter were more abundant in deeper sediment, where 5.7 ± 0.4 × 105 and 5.4 ± 0.9 × 106 16S rRNA gene copies per g of sediment, respectively, were measured. The microbiological and hydrogeological characterization demonstrated that microbial processes at the fractured bedrock–sediment interface were crucial for preventing contaminants reaching the water column, emphasizing the relevance of this critical zone environment for contaminant attenuation.
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影响因子：
4.4
作者：
BOWMAN, JP;JIMENEZ, L;SAYLER, GS
通讯作者：
SAYLER, GS
影响因子：
1.4
作者：
Griebler, C;Mindl, B;Geiger-Kaiser, M
通讯作者：
Geiger-Kaiser, M
影响因子：
4.4
作者：
Amos, Benjamin K.;Sung, Youlboong;Loeffler, Frank E.
通讯作者：
Loeffler, Frank E.
影响因子：
4.2
作者：
Hamonts, Kelly;Ryngaert, Annemie;Dejonghe, Winnie
通讯作者：
Dejonghe, Winnie
影响因子：
1.9
作者：
Key, Katherine C.;Sublette, Kerry L.;Biernacki, Anita
通讯作者：
Biernacki, Anita