Functional characterization of a second pedal peptide/orcokinin-type neuropeptide signaling system in the starfish Asterias rubens.
Functional characterization of a second pedal peptide/orcokinin-type neuropeptide signaling system in the starfish Asterias rubens.
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DOI：
10.1002/cne.24371
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发表时间：
2018-04-01
期刊：
影响因子：
--
通讯作者：
Elphick MR
中科院分区：
文献类型：
--
作者：
Lin M;Egertová M;Zampronio CG;Jones AM;Elphick MR
软体动物足肽（PP）和节肢动物orcokinins（OKs）是神经肽家族的原型，已在几个门中被鉴定。最近，海星肌松弛肽（SMP）被鉴定为海星Patiria pectinifera（棘皮动物门）中的PP/OK-型神经肽。此外，对来自海星Asterias rubens的转录组序列数据的分析揭示了两种PP/OK型前体：SMP型前体（A. Rubens PP样神经肽前体1; ArPPLNP 1）和第二前体（ArPPLNP 2）。我们先前报道了ArPPLNP 1在A.在这里，我们报告了ArPPLNP 2衍生神经肽的第一个功能表征。编码ArPPLNP 2的cDNA的测序显示，它包含11种相关的神经肽（ArPPLN 2a-k），其中几种的结构使用质谱法进行了确认。使用mRNA原位杂交和免疫组织化学分析ArPPLNP 2和来自该前体的神经肽的表达揭示了广泛的分布，包括在桡神经索、口周神经环、消化系统、管足和神经支配的骨间肌中的表达。体外药理学研究表明，ArPPLN 2衍生的神经肽ArPPLN 2 h对管足或体壁相关顶端肌肉的收缩性没有影响，与ArPPLN 1b（ArSMP）对这些制剂的舒张作用形成对比。然而，ArPPLN 2 h确实会引起心脏胃制剂的剂量依赖性舒张，其效力/功效比ArPPLN 1b更大，并且与SALMF酰胺神经肽S2具有相似的效力/功效。总之，ArPPLNP 1和ArPPLNP 2的表达模式存在相似性，但我们的数据也表明来自这两种PP/OK-型前体的神经肽在海星中的作用的专业化。
Molluscan pedal peptides (PPs) and arthropod orcokinins (OKs) are prototypes of a family of neuropeptides that have been identified in several phyla. Recently, starfish myorelaxant peptide (SMP) was identified as a PP/OK‐type neuropeptide in the starfish Patiria pectinifera (phylum Echinodermata). Furthermore, analysis of transcriptome sequence data from the starfish Asterias rubens revealed two PP/OK‐type precursors: an SMP‐type precursor (A. rubens PP‐like neuropeptide precursor 1; ArPPLNP1) and a second precursor (ArPPLNP2). We reported previously a detailed analysis of ArPPLNP1 expression in A. rubens and here we report the first functional characterization ArPPLNP2‐derived neuropeptides. Sequencing of a cDNA encoding ArPPLNP2 revealed that it comprises eleven related neuropeptides (ArPPLN2a‐k), the structures of several of which were confirmed using mass spectrometry. Analysis of the expression of ArPPLNP2 and neuropeptides derived from this precursor using mRNA in situ hybridization and immunohistochemistry revealed a widespread distribution, including expression in radial nerve cords, circumoral nerve ring, digestive system, tube feet and innervation of interossicular muscles. In vitro pharmacology revealed that the ArPPLNP2‐derived neuropeptide ArPPLN2h has no effect on the contractility of tube feet or the body wall‐associated apical muscle, contrasting with the relaxing effect of ArPPLN1b (ArSMP) on these preparations. ArPPLN2h does, however, cause dose‐dependent relaxation of cardiac stomach preparations, with greater potency/efficacy than ArPPLN1b and with similar potency/efficacy to the SALMFamide neuropeptide S2. In conclusion, there are similarities in the expression patterns of ArPPLNP1 and ArPPLNP2 but our data also indicate specialization in the roles of neuropeptides derived from these two PP/OK‐type precursors in starfish.
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影响因子：
3.8
作者：
Jiang H;Kim HG;Park Y
通讯作者：
Park Y
鉴定出充当肌肉松弛剂的新型海星神经肽。
影响因子：
4.7
作者：
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通讯作者：
Park NG
影响因子：
1.5
作者：
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通讯作者：
Flammang, P
影响因子：
2.8
作者：
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通讯作者：
Elphick, MR
影响因子：
2.8
作者：
Hofer, Sabine;Homberg, Uwe
通讯作者：
Homberg, Uwe