Preparation of Graphene Quantum Dots by Visible-Fenton Reaction and Ultrasensitive Label-Free Immunosensor for Detecting Lipovitellin of Paralichthys Olivaceus.
Preparation of Graphene Quantum Dots by Visible-Fenton Reaction and Ultrasensitive Label-Free Immunosensor for Detecting Lipovitellin of Paralichthys Olivaceus.
复制标题
可见光芬顿反应制备石墨烯量子点及超灵敏无标记免疫传感器检测牙鲆脂蛋白
DOI：
10.3390/bios12040246
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发表时间：
2022-04-15
期刊：
影响因子：
5.4
通讯作者：
Wang, Jun
中科院分区：
文献类型：
--
作者：
Yang, Ailing;Su, Yue;Zhang, Zhenzhong;Wang, Huaidong;Qi, Chong;Ru, Shaoguo;Wang, Jun
关键词：
环境雌激素水平的上升正在对水、土壤、野生动物和人类造成负面影响；具有高特异性和敏感性的无标签免疫传感器正在开发中，以测试复杂环境条件下的异质化学品。首次以氧化石墨烯（GO）为前驱体，采用可见fenton催化反应制备了高荧光石墨烯量子点（GQDs）。采用不同的显微和光谱学技术表征了GQDs的物理和化学性质。基于氨基功能化GQDs与抗脂卵磷脂单克隆抗体（Anti-Lv-mAb）和还原氧化石墨烯（rGO）之间的荧光共振能量转移（FRET），建立了一种检测脂卵磷脂（Lv）的超灵敏荧光“on - off”无标记免疫传感器，Lv是一种来自olivaceus的敏感生物标志物，用于检测环境雌激素。该免疫传感器线性检测范围宽（0.001 ~ 1500 ng/mL），检测下限低（LOD, 0.9 pg/mL），灵敏度高（26407.8 CPS/(ng/mL)），对Lv定量具有高选择性和重复性。结果表明，可见芬顿法是一种简单、温和、绿色、高效、通用的制备GQDs的方法，荧光“ON-OFF”免疫传感器是一种易于使用、节省时间、超灵敏、准确的弱雌激素活性检测方法。
The increasing levels of environmental estrogens are causing negative effects on water, soil, wildlife, and human beings; label-free immunosensors with high specificities and sensitivities are being developed to test estrogeneous chemicals in complex environmental conditions. For the first time, highly fluorescent graphene quantum dots (GQDs) were prepared using a visible-Fenton catalysis reaction with graphene oxide (GO) as a precursor. Different microscopy and spectroscopy techniques were employed to characterize the physical and chemical properties of the GQDs. Based on the fluorescence resonance energy transfer (FRET) between amino-functionalized GQDs conjugated with anti-lipovitellin monoclonal antibodies (Anti-Lv-mAb) and reduced graphene oxide (rGO), an ultrasensitive fluorescent “ON-OFF” label-free immunosensor for the detection of lipovitellin (Lv), a sensitive biomarker derived from Paralichthys olivaceus for environmental estrogen, has been established. The immunosensor has a wide linear test range (0.001–1500 ng/mL), a lower limit of detection (LOD, 0.9 pg/mL), excellent sensitivity (26,407.8 CPS/(ng/mL)), and high selectivity and reproducibility for Lv quantification. The results demonstrated that the visible-Fenton is a simple, mild, green, efficient, and general approach to fabricating GQDs, and the fluorescent “ON-OFF” immunosensor is an easy-to-use, time-saving, ultrasensitive, and accurate detection method for weak estrogenic activity.
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影响因子：
8.4
作者：
Adanyi, Nora;Majer-Baranyi, Krisztina;Szekacs, Andras
通讯作者：
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DOI：
10.3390/s18124124
发表时间：
2018-11-24
期刊：
Sensors (Basel, Switzerland)
影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
Lee, Jihoon
DOI：
10.1016/s1532-0456(01)00194-6
发表时间：
2001-07-01
影响因子：
3.9
作者：
Fenske, M;van Aerle, R;Segner, H
通讯作者：
Segner, H
影响因子：
4.1
作者：
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通讯作者：
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