Variable expression of surface-exposed polymorphic membrane proteins in in vitro-grown Chlamydia trachomatis.
Variable expression of surface-exposed polymorphic membrane proteins in in vitro-grown Chlamydia trachomatis.
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DOI：
10.1111/j.1462-5822.2009.01389.x
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发表时间：
2010-02
影响因子：
3.4
通讯作者：
Bavoil PM
中科院分区：
文献类型：
--
作者：
Tan C;Hsia RC;Shou H;Carrasco JA;Rank RG;Bavoil PM
通过检测沙眼衣原体各亚型PMP在体外培养的沙眼衣原体中PMP亚型的表达，验证了PmPA-PmpI在沙眼衣原体感染患者中的假想可变表达。使用一组单特异性的多克隆和单抗，通过差示免疫荧光(IF)和免疫金标记法显示每一亚型的PMPs在有或没有多聚甲醛固定的包裹体的洗涤剂渗透的情况下的表面暴露。虽然在发育后期检测到每个PMP基因的特定转录本，但如果与PMP亚型特异性抗体的实验表明，单个感染中的许多包涵体不表达给定亚型的PMP。共表达实验表明，在非表达的包涵体中，PMP基因相互独立地被关闭，即不同的PMP被关闭。总体而言，这些研究证实，在体外培养的沙眼衣原体中，存在一种有效的关闭机制，独立地影响PMP基因家族的每个成员的表达。与其他类似的细菌病原体基因家族一样，沙眼衣原体的PMP基因家族可能在面对宿主适应性免疫反应时发挥着关键的双重功能，即高度适应性的毒力因子，并提供抗原多样性。
The hypothesized variable expression of polymorphic membrane proteins (PmpA-PmpI) in Chlamydia trachomatis-infected patients was tested by examination of the expression of each Pmp subtype in in vitro-grown C. trachomatis. A panel of monospecific polyclonal and monoclonal antibodies was used to demonstrate surface exposure of Pmps of each subtype by differential immunofluorescence (IF) with and without prior detergent permeabilization of paraformaldehyde-fixed inclusions and for selected Pmps by immunogold labeling. Although specific transcript was detected for each pmp gene late in development, IF experiments with Pmp subtype-specific antibodies reveal that a number of inclusions in a single infection do not express Pmps of a given subtype. Co-expression experiments suggest that pmp genes are shut off independently from one another in non-expressing inclusions, i.e. different inclusions are switched off for different Pmps. Overall, these studies establish the existence of an efficient shutoff mechanism independently affecting the expression of each member of the pmp gene family in in vitro-grown C. trachomatis. Like other paralogous gene families of bacterial pathogens, the pmp gene family of C. trachomatis may serve the critical dual function of a highly adaptable virulence factor also providing antigenic diversity in the face of the host adaptive immune response.
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影响因子：
2.1
作者：
Mygind, PH;Christiansen, G;Birkelund, S
通讯作者：
Birkelund, S
影响因子：
6.4
作者：
Niessner, A;Kaun, C;Wojta, J
通讯作者：
Wojta, J
影响因子：
14.9
作者：
Read, TD;Brunham, RC;Fraser, CM
通讯作者：
Fraser, CM
影响因子：
3.2
作者：
Gomes, JP;Nunes, A;Dean, D
通讯作者：
Dean, D
影响因子：
3.1
作者：
CALDWELL, HD;KROMHOUT, J;SCHACHTER, J
通讯作者：
SCHACHTER, J