Repeated superovulation increases the risk of osteoporosis and cardiovascular diseases by accelerating ovarian aging in mice
Repeated superovulation increases the risk of osteoporosis and cardiovascular diseases by accelerating ovarian aging in mice
复制标题
反复超排卵会加速小鼠卵巢衰老，从而增加骨质疏松症和心血管疾病的风险
DOI：
10.18632/aging.101449
复制
发表时间：
2018-05
期刊：
影响因子：
5.2
通讯作者：
Wang Shixuan
中科院分区：
文献类型：
--
作者：
Zhang Jinjin;Lai Zhiwen;Shi Liangyan;Tian Yong;Luo Aiyue;Xu Zheyuan;Ma Xiangyi;Wang Shixuan
超排卵程序和辅助生殖技术已被广泛用于治疗有不孕问题的夫妇。虽然超排卵程序通常是安全的，但与一系列并发症有关，如卵巢过度刺激综合征，血栓栓塞和附件扭转。长期重复超数排卵在卵巢衰老中的作用，特别是在相关疾病如骨质疏松症和心血管疾病中的作用尚不清楚。在这项研究中，我们试图确定重复超排卵10个周期的治疗与孕马血清促性腺激素/人绒毛膜促性腺激素可能会影响卵巢储备，卵巢功能，骨密度和心脏功能。原始卵泡池的大小、抗苗勒管激素的表达、激素水平和生育状况反映了卵巢储备和功能。此外，我们分别通过microCT和心血管超声检查骨密度和心脏功能。重复超排后，原始卵泡池的大小和抗苗勒管激素的表达随雌、孕激素浓度的增加而沿着减少。重复超数排卵的小鼠生育力和繁殖力明显下降。此外，骨密度和心脏射血分数均显著降低。这些结果表明，重复超数排卵可能会通过加速卵巢老化而增加骨质疏松症和心血管疾病的风险。
Superovulation procedures and assisted reproductive technologies have been widely used to treat couples who have infertility problems. Although generally safe, the superovulation procedures are associated with a series of complications, such as ovarian hyper-stimulation syndrome, thromboembolism, and adnexal torsion. The role of long-term repeated superovulation in ovarian aging and especially in associated disorders such as osteoporosis and cardiovascular diseases is still unclear. In this study, we sought to determine if repeated superovulation by ten cycles of treatment with pregnant mare serum gonadotropin/human chorionic gonadotropin could affect ovarian reserve, ovarian function, bone density and heart function. Ovarian reserve and function were reflected by the size of the primordial follicle pool, anti-Mullerian hormone expressions, hormone levels and fertility status. Furthermore, we examined bone density and heart function by microCT and cardiovascular ultrasonography, respectively. After repeated superovulation, the size of the primordial follicle pool and the expression of anti-mullerian hormone decreased, along with the concentrations of estrogen and progesterone. Mice exposed to repeated superovulation showed an obvious decrease in fertility and fecundity. Furthermore, both bone density and heart ejection fraction significantly decreased. These results suggest that repeated superovulation may increase the risk of osteoporosis and cardiovascular diseases by accelerating ovarian aging.
登录
查看更多内容
影响因子：
7.4
作者：
Miyamoto, Kaori;Sato, Eisuke F.;Inoue, Masayasu
通讯作者：
Inoue, Masayasu
影响因子：
6.7
作者：
Soules, Michael R.;Sherman, Sherry;Woods, Nancy
通讯作者：
Woods, Nancy
影响因子：
2.4
作者：
MUKUMOTO, S;MORI, K;ISHIKAWA, H
通讯作者：
ISHIKAWA, H
影响因子：
6.1
作者：
J. Blerkom;P. Davis
通讯作者：
J. Blerkom;P. Davis
影响因子：
2.5
作者：
Makabe, Sayoko;Naguro, Tomonori;Stallone, Tiziana
通讯作者：
Stallone, Tiziana