Development of nanoparticle-based magnetic resonance colonography.
Development of nanoparticle-based magnetic resonance colonography.
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DOI：
10.1002/mrm.22654
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发表时间：
2011-03
影响因子：
3.3
通讯作者：
Zhang, Shizheng
中科院分区：
文献类型：
--
作者：
Sun, Jihong;Zheng, Weiliang;Zhang, Hui;Wu, Tao;Yuan, Hong;Yang, Xiaoming;Zhang, Shizheng
关键词：
This study was to develop a novel method of nanoparticle-based MR colonography. Two types of solid lipid nanoparticles (SLNs) were synthesized with loading of (a) gadolinium-diethylenetriaminepenta-acetic-acid (Gd-DTPA) to construct Gd-SLNs as an MR T1 contrast agent; and (b) otcadecylamine-fluorescein-isothiocyanate (ODA-FITC) to construct Gd-FITC-SLNs for histologic confirmation of MR findings. Through an in vitro experiment, we first evaluated the size distribution and Gd-DTPA entrapment efficiency of these SLNs. The SLNs displayed a size distribution of 50–300 nm and a Gd-DTPA entrapment efficiency of 56%. For in vivo validation, thirty mice were divided into five groups, each of which was administered a transrectal enema using: (i) Gd-SLNs (n=6); (ii) Gd-FITC-SLNs (n=6); (iii) blank SLNs (n=6); (iv) Gd-DTPA (n=6); and (v) water (n=6). T1-weighted FLAIR MRI was then performed on mice after transrectal infusion of Gd-SLNs or Gd-FITC-SLNs, which demonstrated bright enhancement of the colonic walls, with decrease in T1 relaxation time. When Gd-FITC-SLNs were delivered, green fluorescent spots were visualized in both the extracellular space and the cytoplasm through colonic walls under confocal microscopy and fluorescence microscopy. This study establishes the “proof-of-principle” of a new imaging technique, called “nanoparticle-based MR colonography,” which may provide a useful imaging tool for the diagnosis of colorectal diseases.
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影响因子：
5
作者：
WEINMANN, HJ;BRASCH, RC;WESBEY, GE
通讯作者：
WESBEY, GE
影响因子：
3.7
作者：
SANDERS, E;ASHWORTH, CT
通讯作者：
ASHWORTH, CT
影响因子：
4.8
作者：
Nagata, Koichi;Singh, Anand Kumar;Yoshida, Hiroyuki
通讯作者：
Yoshida, Hiroyuki
影响因子：
3.3
作者：
Chen, HH;Le Visage, C;Yang, XM
通讯作者：
Yang, XM
影响因子：
3.3
作者：
Mamisch, Tallal Charles;Dudda, Marcel;Kim, Young-Jo
通讯作者：
Kim, Young-Jo