Visualization of protease activity in vivo using an activatable photo-acoustic imaging probe based on CuS nanoparticles.
Visualization of protease activity in vivo using an activatable photo-acoustic imaging probe based on CuS nanoparticles.
复制标题
使用基于 CuS 纳米颗粒的可激活光声成像探针实现体内蛋白酶活性的可视化
作者：
Yang K;Zhu L;Nie L;Sun X;Cheng L;Wu C;Niu G;Chen X;Liu Z
Herein, we for the first time report a novel activatable photoacoustic (PA) imaging nano-probe for in vivo detection of cancer-related matrix metalloproteinases (MMPs). A black hole quencher 3 (BHQ3) which absorbs red light is conjugated to near-infrared (NIR)-absorbing copper sulfide (CuS) nanoparticles via a MMP-cleavable peptide linker. The obtained CuS-peptide-BHQ3 (CPQ) nano-probe exhibits two distinctive absorption peaks at 630 nm and 930 nm. Inside the tumor microenviorment where MMPs present, the MMP-sensitive peptide would be cleaved, releasing BHQ3 from the CuS nanoparticles, the former of which as a small molecule is then rapidly cleared out from the tumor, whereas the latter of which as large nanoparticles would retain inside the tumor for a much longer period of time. As the result, the PA signal at 680 nm which is contributed by BHQ3 would be quickly diminished while that at 930 nm would be largely retained. The PA signal ratio of 680 nm / 930 nm could thus serve as an in vivo indicator of MMPs activity inside the tumor. Our work presents a novel strategy of in vivo sensing of MMPs based on PA imaging, which should offer remarkably improved detection depth compared with traditional optical imaging techniques.
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用于活体小鼠高灵敏度和多重研究的增强型光声成像剂系列。
影响因子：
17.1
作者：
de la Zerda A;Bodapati S;Teed R;May SY;Tabakman SM;Liu Z;Khuri-Yakub BT;Chen X;Dai H;Gambhir SS
通讯作者：
Gambhir SS
影响因子：
38.3
作者：
通讯作者：
--
影响因子：
8
作者：
Ala-aho, R;Ahonen, M;Kähäri, VM
通讯作者：
Kähäri, VM
影响因子：
10.8
作者：
Lee S;Ryu JH;Park K;Lee A;Lee SY;Youn IC;Ahn CH;Yoon SM;Myung SJ;Moon DH;Chen X;Choi K;Kwon IC;Kim K
通讯作者：
Kim K
影响因子：
17.1
作者：
Ku, Geng;Zhou, Min;Song, Shaoli;Huang, Qian;Hazle, John;Li, Chun
通讯作者：
Li, Chun