Desert truffle genomes reveal their reproductive modes and new insights into plant-fungal interaction and ectendomycorrhizal lifestyle.
Desert truffle genomes reveal their reproductive modes and new insights into plant-fungal interaction and ectendomycorrhizal lifestyle.
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DOI：
10.1111/nph.17044
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发表时间：
2021-03
期刊：
影响因子：
--
通讯作者：
Morte A
中科院分区：
文献类型：
--
作者：
Marqués-Gálvez JE;Miyauchi S;Paolocci F;Navarro-Ródenas A;Arenas F;Pérez-Gilabert M;Morin E;Auer L;Barry KW;Kuo A;Grigoriev IV;Martin FM;Kohler A;Morte A
沙漠松露是一种可食用的次生真菌，与肉桂科植物形成外生菌根共生关系。关于这些真菌的繁殖模式和驱动外生菌根相互作用的分子机制的知识还很缺乏。高度重视的可食用沙漠松露Terfezia clveryi Chatin和Tirmania Nivea Trappe的基因组已经被测序，并与其他Pezizmyces进行了比较。研究了水分充足和干旱胁迫植物上的红花木霉菌根在促进细胞内定植时的转录本。我们已经确定了沙漠块菌中与有性繁殖相关的真菌基因以及沙漠块菌特有的基因组和分泌学特征，例如，一大组未知Pfam结构域的基因家族的扩展，以及一些在共生中存在差异调节的物种或沙漠块菌特有的小分泌蛋白。发现了一组核心植物基因，包括碳水化合物、脂肪代谢和防御相关基因，在这两种条件下在菌根中差异表达。我们的结果突出了沙漠松露相对于其他菌根真菌的独特性，同时提供了第一次对干旱条件下沙漠松露内从外向内共生转换所涉及的植物和真菌决定因素的一瞥。
Desert truffles are edible hypogeous fungi forming ectendomycorrhizal symbiosis with plants of Cistaceae family. Knowledge about the reproductive modes of these fungi and the molecular mechanisms driving the ectendomycorrhizal interaction is lacking. Genomes of the highly appreciated edible desert truffles Terfezia claveryi Chatin and Tirmania nivea Trappe have been sequenced and compared with other Pezizomycetes. Transcriptomes of T. claveryi × Helianthemum almeriense mycorrhiza from well‐watered and drought‐stressed plants, when intracellular colonizations is promoted, were investigated. We have identified the fungal genes related to sexual reproduction in desert truffles and desert‐truffles‐specific genomic and secretomic features with respect to other Pezizomycetes, such as the expansion of a large set of gene families with unknown Pfam domains and a number of species or desert‐truffle‐specific small secreted proteins differentially regulated in symbiosis. A core set of plant genes, including carbohydrate, lipid‐metabolism, and defence‐related genes, differentially expressed in mycorrhiza under both conditions was found. Our results highlight the singularities of desert truffles with respect to other mycorrhizal fungi while providing a first glimpse on plant and fungal determinants involved in ecto to endo symbiotic switch that occurs in desert truffle under dry conditions.
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影响因子：
10.7
作者：
Castresana, J
通讯作者：
Castresana, J
影响因子：
14.9
作者：
Grigoriev IV;Nikitin R;Haridas S;Kuo A;Ohm R;Otillar R;Riley R;Salamov A;Zhao X;Korzeniewski F;Smirnova T;Nordberg H;Dubchak I;Shabalov I
通讯作者：
Shabalov I
影响因子：
3.9
作者：
Baptista, Paula;Martins, Anabela;Lino-Neto, Teresa
通讯作者：
Lino-Neto, Teresa
影响因子：
7.3
作者：
Fernandez Del-Saz, Nestor;Romero-Munar, Antonia;Ribas-Carbo, Miquel
通讯作者：
Ribas-Carbo, Miquel
影响因子：
10.7
作者：
Katoh K;Standley DM
通讯作者：
Standley DM