Maximum fluorescence and electron transport kinetics determined by light-induced fluorescence transients (LIFT) for photosynthesis phenotyping.
Maximum fluorescence and electron transport kinetics determined by light-induced fluorescence transients (LIFT) for photosynthesis phenotyping.
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DOI：
10.1007/s11120-018-0594-9
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发表时间：
2019-05
影响因子：
3.7
通讯作者：
Muller O
中科院分区：
文献类型：
--
作者：
Keller B;Vass I;Matsubara S;Paul K;Jedmowski C;Pieruschka R;Nedbal L;Rascher U;Muller O
当在波动的环境中测量大量植物数量时，光合表型需要快速表征动态性状。在这里，我们评估了光诱导荧光瞬态（LIFT）方法在0.6 m距离内快速产生荧光参数的能力。在控制条件下，当电子传递受到缺氧条件或化学抑制剂的损害时，菠菜叶片和分离的类囊体的LIFT与常规叶绿素荧光（ChlF）参数非常接近。快速重复率（Fm−FRR）闪光诱导的ChlF从最小荧光（Fo）上升到最大荧光（QA）主要是光系统II中初级电子受体的还原。用ChlF在0.65 ms （Fr1）和120 ms （Fr2）相的弛豫来定量QA−的随后再氧化。当电子传递受损时，QA−（Fr1/Fv，其中Fv = Fm−FRR−Fo）的再氧化效率降低，而光系统II的量子效率（Fv/Fm）通常没有显著影响。LIFT的ChlF弛豫与独立的其他方法相似。在增加光强的情况下，Fr2’/Fq’（其中Fr2’和Fq’分别代表光适应状态下的Fr2和Fv）几乎没有受到影响，而光系统II的工作效率（Fq’/Fm’）由于非光化学猝灭而降低。Fm−FRR显著低于多次翻转（Fm−MT）闪光诱导的ChlF最大值。然而，两次闪光产生的Fv/Fm和Fq ‘ /Fm ’是高度相关的。LIFT方法用电子输运效率的信息补充了Fv/Fm。原位和远距离测量有助于高通量和自动化表型的应用。本文的在线版本（10.1007/s11120-018-0594-9）包含补充材料，仅供授权用户使用。
Photosynthetic phenotyping requires quick characterization of dynamic traits when measuring large plant numbers in a fluctuating environment. Here, we evaluated the light-induced fluorescence transient (LIFT) method for its capacity to yield rapidly fluorometric parameters from 0.6 m distance. The close approximation of LIFT to conventional chlorophyll fluorescence (ChlF) parameters is shown under controlled conditions in spinach leaves and isolated thylakoids when electron transport was impaired by anoxic conditions or chemical inhibitors. The ChlF rise from minimum fluorescence (Fo) to maximum fluorescence induced by fast repetition rate (Fm−FRR) flashes was dominated by reduction of the primary electron acceptor in photosystem II (QA). The subsequent reoxidation of QA− was quantified using the relaxation of ChlF in 0.65 ms (Fr1) and 120 ms (Fr2) phases. Reoxidation efficiency of QA− (Fr1/Fv, where Fv = Fm−FRR − Fo) decreased when electron transport was impaired, while quantum efficiency of photosystem II (Fv/Fm) showed often no significant effect. ChlF relaxations of the LIFT were similar to an independent other method. Under increasing light intensities, Fr2′/Fq′ (where Fr2′ and Fq′ represent Fr2 and Fv in the light-adapted state, respectively) was hardly affected, whereas the operating efficiency of photosystem II (Fq′/Fm′) decreased due to non-photochemical quenching. Fm−FRR was significantly lower than the ChlF maximum induced by multiple turnover (Fm−MT) flashes. However, the resulting Fv/Fm and Fq′/Fm′ from both flashes were highly correlated. The LIFT method complements Fv/Fm with information about efficiency of electron transport. Measurements in situ and from a distance facilitate application in high-throughput and automated phenotyping. The online version of this article (10.1007/s11120-018-0594-9) contains supplementary material, which is available to authorized users.
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影响因子：
3.7
作者：
Ananyev, G;Dismukes, GC
通讯作者：
Dismukes, GC
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