Evaluation of the antibacterial effect of Epigallocatechin gallate on the major pathogens of canine periodontal disease and therapeutic effects on periodontal disease mice.
Evaluation of the antibacterial effect of Epigallocatechin gallate on the major pathogens of canine periodontal disease and therapeutic effects on periodontal disease mice.
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DOI：
10.3389/fmicb.2023.1329772
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发表时间：
2023
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
--
作者：
牙周病（PD）是犬的一种常见口腔疾病，治疗选择有限。口腔细菌可能通过种间接触从犬传播给人类，这构成了人畜共患感染的风险。表没食子儿茶素没食子酸酯（EGCG）是绿色茶多酚中的主要儿茶素，具有有效对抗人类PD的抗菌特性。鉴于犬和人PD之间的临床相似性，本研究探讨了采用EGCG作为犬PD治疗剂的可行性。首先，对中国犬PD相关细菌感染数据进行了调查和统计分析。对犬PD的主要致病菌进行分离培养，并对EGCG的体外抗菌效果进行评价。进一步验证EGCG对小鼠PD模型的体内治疗作用。高通量16 S rRNA基因测序鉴定出卟啉单胞菌属、梭杆菌属、密螺旋体属、莫拉氏菌属和二氧化碳噬细胞菌属是我国健康犬牙龈沟液（GCF）样本中区分PD的菌属。从72份犬龈沟液标本中分离出92株临床菌株，包括古莱卟啉单胞菌、中间普雷沃菌、麦氏卟啉单胞菌等22个种。EGCG的最低抑菌浓度（MIC）为0.019 ~ 1.25 mg/mL。在7天的口腔粘膜施用中等剂量的EGCG（0.625 mg/mL）后，PD小鼠中牙周微生物的丰度显著降低。这种干预改善了牙槽骨丢失，将牙骨质-釉质连接部至牙槽骨嵴的平均距离（CEJ-ABC）从0.306 mm ± 0.050 mm降低至0.161 mm ± 0.026 mm。此外，EGCG（0.3125 mg/mL）显著下调PD小鼠血清中炎症因子IL-6的表达。我们的研究表明，表没食子酸没食子酸酯对犬PD中流行的细菌古雷氏杆菌具有显着的抗菌作用。此外，表没食子儿茶素没食子酸酯表现出抗炎特性，并证明有效解决PD小鼠模型中的骨丢失。这些发现共同表明了EGCG在治疗犬PD中的治疗潜力。本研究的结果提供了有价值的数据，为进一步探索和筛选替代抗生素药物以推进犬PD的管理奠定了基础。
Periodontal disease (PD) is a prevalent oral affliction in canines, with limited therapeutic options available. The potential transmission of oral bacteria from canines to humans through inter-species contact poses a risk of zoonotic infection. Epigallocatechin gallate (EGCG), the principal catechin in green tea polyphenols, exhibits antibacterial properties effective against human PD. Given the clinical parallels between canine and human PD, this study explores the feasibility of employing EGCG as a therapeutic agent for canine PD. Initially, a survey and statistical analysis of bacterial infection data related to canine PD in China were conducted. Subsequently, the primary pathogenic bacteria of canine PD were isolated and cultivated, and the in vitro antibacterial efficacy of EGCG was assessed. Furthermore, verify the therapeutic effect of EGCG on a mouse PD model in vivo. The high-throughput 16S rRNA gene sequencing identified Porphyromonas, Fusobacterium, Treponema, Moraxella, and Capnocytophaga as the genera that distinguishing PD from healthy canines’ gingival crevicular fluid (GCF) samples in China. The anaerobic culture and drug susceptibility testing isolated a total of 92 clinical strains, representing 22 species, from 72 canine GCF samples, including Porphyromonas gulae, Prevotella intermedia, Porphyromonas macacae, etc. The minimum inhibitory concentration (MIC) ranging of EGCG was from 0.019 to 1.25 mg/mL. Following a 7 days oral mucosal administration of medium-dose EGCG (0.625 mg/mL), the abundance of periodontal microorganisms in PD mice significantly decreased. This intervention ameliorated alveolar bone loss, reducing the average cementoenamel junction to the alveolar bone crest (CEJ-ABC) distance from 0.306 mm ± 0.050 mm to 0.161 mm ± 0.026 mm. Additionally, EGCG (0.3125 mg/mL) markedly down-regulated the expression of inflammatory factor IL-6 in the serum of PD mice. Our research demonstrates the significant antibacterial effects of EGCG against the prevalent bacterium P. gulae in canine PD. Moreover, EGCG exhibits anti-inflammatory properties and proves effective in addressing bone loss in a PD mouse model. These findings collectively suggest the therapeutic potential of EGCG in the treatment of canine PD. The outcomes of this study contribute valuable data, laying the foundation for further exploration and screening of alternative antibiotic drugs to advance the management of canine PD.
影响因子：
4
作者：
Shimizu, Mamu;Miyawaki, Shingo;Kuroda, Taishin;Umeta, Miyu;Kawabe, Mifumi;Watanabe, Kazuhiro
通讯作者：
Watanabe, Kazuhiro
（ - ） - 表瓜蛋白3-gallate对卟啉单胞菌脂肪含糖刺激的成纤维细胞和干细胞源自人牙周韧带的抗炎作用。
影响因子：
1.9
作者：
Jung IH;Lee DE;Yun JH;Cho AR;Kim CS;You YJ;Kim SJ;Choi SH
通讯作者：
Choi SH