Wavelike propagation of quorum activation through a spatially distributed bacterial population under natural regulation
Wavelike propagation of quorum activation through a spatially distributed bacterial population under natural regulation
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在自然调节下通过空间分布的细菌群体进行群体激活的波状传播
DOI：
10.1088/1478-3975/ac02ac
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发表时间：
2021
期刊：
影响因子：
2
通讯作者：
Hagen, Stephen J
中科院分区：
文献类型：
--
作者：
Patel, Keval;Rodriguez, Coralis;Stabb, Eric V;Hagen, Stephen J
许多细菌使用称为自诱导物的可扩散信息素信号进行交流。当自诱导物浓度达到阈值时，这需要最小种群密度或“定群”，细菌激活特定的基因调控途径。自诱导物的简单扩散可以激活位于离分泌源相当远的细胞中的群体依赖性途径。然而，模型预测，自体诱导物扩散，加上自体诱导物合成中的正反馈调节，也可以通过以恒定速度移动作为自我维持的前沿，使群体调节行为在种群中更快地传播。在这里，我们表明，这样的繁殖可以发生在一个人口的细菌，其法定途径下运行自己的自然调节。我们发现，在未搅拌的群体中的费氏弧菌，在一个位置的自诱导物的引入触发了天然生物发光的波浪状旅行前。波前以一个明确的速度移动，大约为2.5 mm h− 1，最终超过了初始刺激的较慢扩散扩散。与建模预测一致，波传播直到生长后期，此时由于基础自诱导物的产生而发生群体范围的激活。随后的几轮波，包括反向传播的波，也可以在自然调节下的费氏弧菌生长后期观察到。使用工程，乳糖依赖性菌株，我们表明，当地的刺激比autoinducers也可以引起自我维持，传播的反应。我们的数据表明，通过简单的群体信号数学模型预测的波浪状动力学很容易在细菌种群自身的自然调节下检测到，并且其他更复杂的旅行现象也存在。因为行波可以大大提高宏观距离上细胞间通信的效率，我们的数据表明，非常有效的通信模式在距离上可用于非混合的群体V. fischeri和其他微生物。
Many bacteria communicate using diffusible pheromone signals known as autoinducers. When the autoinducer concentration reaches a threshold, which requires a minimum population density or'quorum', the bacteria activate specific gene regulatory pathways. Simple diffusion of autoinducer can activate quorum-dependent pathways in cells that are located at substantial distances from the secreting source. However, modeling has predicted that autoinducer diffusion, coupled with positive feedback regulation in autoinducer synthesis, could also allow a quorum-regulated behavior to spread more rapidly through a population by moving as a self-sustaining front at constant speed. Here we show that such propagation can occur in a population of bacteria whose quorum pathway operates under its own natural regulation. We find that in unstirred populations of Vibrio fischeri, introduction of autoinducer at one location triggers a wavelike traveling front of natural bioluminescence. The front moves with a well-defined speed∼ 2.5 mm h− 1, eventually outrunning the slower diffusional spreading of the initial stimulus. Consistent with predictions from modeling, the wave travels until late in growth, when population-wide activation occurs due to basal autoinducer production. Subsequent rounds of waves, including waves propagating in the reverse direction, can also be observed late in the growth of V. fischeri under natural regulation. Using an engineered, lac-dependent strain, we show that local stimuli other than autoinducers can also elicit a self-sustaining, propagating response. Our data show that the wavelike dynamics predicted by simple mathematical models of quorum signaling are readily detected in bacterial populations functioning under their own natural regulation, and that other, more complex traveling phenomena are also present. Because a traveling wave can substantially increase the efficiency of intercellular communication over macroscopic distances, our data indicate that very efficient modes of communication over distance are available to unmixed populations of V. fischeri and other microbes.
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DOI：
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