Feasibility of digitally stained multimodal confocal mosaics to simulate histopathology.
Feasibility of digitally stained multimodal confocal mosaics to simulate histopathology.
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DOI：
10.1117/1.3149853
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发表时间：
2009-05
影响因子：
3.5
通讯作者：
Gareau DS
中科院分区：
文献类型：
--
作者：
Gareau DS
吖啶橙染色组织活检的荧光共焦镶嵌显微镜已被证明可以准确识别肿瘤，总体灵敏度为 96.6%，特异性为 89.2%。然而，荧光仅显示类似于组织病理学中的苏木精的核细节，而不显示胶原或细胞质，这可能提供类似于组织病理学中使用的伊红的必要的阴性对比信息。反射模式对比度对胶原蛋白和细胞质敏感，无需染色。为了进一步提高灵敏度和特异性，数字染色共焦马赛克将共焦荧光和反射图像结合在多模态伪彩色图像中，以模仿苏木精和伊红的组织病理学外观，并促进将共焦显微镜引入临床领域。
Fluorescence confocal mosaicing microscopy of tissue biopsies stained with acridine orange has been shown to accurately identify tumors and with an overall sensitivity of 96.6% and specificity of 89.2%. However, fluorescence shows only nuclear detail similar to hematoxylin in histopathology and does not show collagen or cytoplasm, which may provide necessary negative contrast information similar to eosin used in histopathology. Reflectance mode contrast is sensitive to collagen and cytoplasm without staining. To further improve sensitivity and specificity, digitally stained confocal mosaics combine confocal fluorescence and reflectance images in a multimodal pseudo-color image to mimic the appearance of histopathology with hematoxylin and eosin and facilitate the introduction of confocal microscopy into the clinical realm.
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