Metagenomic insights into dissemination of antibiotic resistance across bacterial genera in wastewater treatment.
Metagenomic insights into dissemination of antibiotic resistance across bacterial genera in wastewater treatment.
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DOI：
10.1016/j.chemosphere.2021.129563
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发表时间：
2021-05
期刊：
影响因子：
8.8
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Cheng X;Xu J;Smith G;Zhang Y
本研究的目的是评估传统的污水处理工艺，包括二级处理和氯化去除抗生素耐药基因（ARGs）和抗生素耐药菌（ARB）的影响，并评估ARGs与其潜在的主机在每个处理过程中的关联。结果表明，亚抑菌浓度（< 8 mg/L）的氯化处理可使消毒出水中ARB数量增加; qPCR分析表明，二次处理增加了剩余细菌中ARG的相对丰度，而消毒有效地降低了这些基因的相对丰度。宏基因组分析显示，一个显着的转变，主要细菌属窝藏ARG。沿着处理列，48，95和80属被确定为ARG载体的一级出水中，二级出水中，消毒出水，分别。研究还发现，二次处理增加了潜在ARG宿主的多样性，而二次处理和氯化都扩大了一些ARG在属水平上的宿主范围，这可能是由于抗生素耐药性通过水平转移在细菌属间传播。这项研究强调了越来越多的关注，污水处理厂（WWTPs）可能会传播ARGs，通过将这种影响与特定的处理阶段相关联，并将ARGs与其细菌宿主相关联。
The aim of this study was to evaluate the impacts of conventional wastewater treatment processes including secondary treatment and chlorination on the removal of antibiotic resistance genes (ARGs) and antibiotic resistant bacteria (ARB), and to assess the association of ARGs with their potential hosts in each treatment process. The results showed chlorination with subinhibitory concentration (< 8 mg/L) resulted in an increased ARB number in the disinfection effluent. qPCR analysis indicated secondary treatment increased relative abundance of ARGs in remaining bacteria whereas disinfection reduced the relative abundance of those genes effectively. Metagenomic analysis revealed a significant shift of dominating bacterial genera harboring ARGs. Along the treatment train, 48, 95 and 80 genera were identified to be the ARG carriers in primary effluent, secondary effluent, and disinfection effluent, respectively. It was also found that secondary treatment increased the diversity of potential ARG hosts while both secondary treatment and chlorination broadened the host range of some ARGs at the genus level, which may be attributed to the spread of antibiotic resistance across bacterial genera through horizontal transfer. This study highlights the growing concerns that wastewater treatment plants (WWTPs) may disseminate ARGs by associating this effect to specific treatment stages and by correlating ARGs with their bacterial hosts.
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