Strong anion-exchange liquid chromatography coupled with isotope ratio mass spectrometry using a Liquiface interface.
Strong anion-exchange liquid chromatography coupled with isotope ratio mass spectrometry using a Liquiface interface.
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使用 Liquiface 接口进行强阴离子交换液相色谱与同位素比质谱联用。
DOI：
10.1002/rcm.4572
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Morrison DJ
中科院分区：
文献类型：
--
作者：
Morrison DJ
引入液相色谱-同位素比质谱联用（LC/IRMS）作为测量非挥发性分析物中同位素比的分析工具，在一定程度上简化了从样品采集到分析的分析周期，主要是由于避免了气相色谱/燃烧/同位素比质谱联用（GC/C/IRMS）所需的大量样品处理和衍生化。在这里，我们测试了仅使用第二种商用接口Liquiface将强阴离子交换耦合到IRMS的性能。该系统根据安装规范进行了修改，以提高接口的峰分辨率，并保持色谱柱与质谱仪之间的峰分离。通过测定从碳水化合物分离获得的灵敏度、准确度和精密度来评估系统性能。改进后的系统性能令人满意，保持了色谱柱与质谱仪之间的峰分离度。该方法的灵敏度为150 ng，精密度<0.3‰。通过与离线技术的相关性确定，在界面中保持准确度，得到回归系数r2= 0.98，斜率为0.99。对7种单糖的平均精密度为0.36‰。碳酸盐去除装置的集成限制了与洗脱液梯度相关的质谱仪中背景碳扰动的影响，并且使用Liquiface成功实现了强阴离子交换色谱与IRMS的耦合。版权所有© 2010约翰威利父子有限公司.
The introduction of liquid chromatography coupled with isotope ratio mass spectrometry (LC/IRMS) as an analytical tool for the measurement of isotope ratios in non‐volatile analytes has somewhat simplified the analytical cycle from sample collection to analysis mainly due to the avoidance of the extensive sample processing and derivatisation that were necessary for gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS). Here we test the performance of coupling strong anion exchange to IRMS using only the second commercially available interface; the Liquiface.The system was modified from installation specification to improve peak resolution in the interface and maintain peak separation from the column to the mass spectrometer. The system performance was assessed by the determination of sensitivity, accuracy and precision attained from carbohydrate separations. The system performed satisfactorily after modifications, resulting in maintenance of peak resolution from column to mass spectrometer. The sensitivity achieved suggested that ∼150 ng carbon could be analysed with acceptable precision (<0.3‰). Accuracy was maintained in the interface as determined by correlation with offline techniques, resulting in regression coefficient of r2= 0.98 and a slope of 0.99. The average precision achieved for the separation of seven monosaccharides was 0.36‰. The integration of a carbonate removal device limited the effect of background carbon perturbations in the mass spectrometer associated with eluent gradients, and the coupling of strong anion‐exchange chromatography with IRMS was successfully achieved using the Liquiface. Copyright © 2010 John Wiley & Sons, Ltd.
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