Changes in membrane conductance play a pathogenic role in osmotic glial cell swelling in detached retinas.
Changes in membrane conductance play a pathogenic role in osmotic glial cell swelling in detached retinas.
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膜电导的变化在视网膜脱离的渗透性神经胶质细胞肿胀中发挥致病作用。
DOI：
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发表时间：
2006
影响因子：
6
通讯作者：
A. Bringmann
中科院分区：
文献类型：
--
作者：
A. Wurm;T. Pannicke;I. Iandiev;E. Bühner;U. Pietsch;A. Reichenbach;P. Wiedemann;S. Uhlmann;A. Bringmann
神经视网膜与色素上皮的脱离可能与组织水肿有关;然而，液体积聚的机制尚不清楚。由于视网膜脱离通常不伴有血管渗漏，我们研究了猪视网膜实验性脱离后视网膜神经胶质（Müller）细胞的渗透性肿胀特征是否改变。渗透压，诱导应用低渗浴解决方案的视网膜切片，造成肿胀的缪勒细胞体在7天的脱离视网膜，但没有肿胀是可诱导的控制视网膜切片。原位局部脱离周围视网膜区域的Müller细胞胞体切片也显示出渗透性肿胀，尽管幅度较小。渗透性Müller细胞肿胀的幅度与K+电导的降低相关，表明两种胶质细胞改变之间存在因果关系。与Müller细胞肿胀有关的其他因素是炎症介质和氧化应激。我们认为，通过Müller细胞的离子和水运输的失调可能会损害视网膜组织的液体吸收，导致脱离后慢性液体积聚。这些知识可能会导致更好地了解视网膜脱离后视网膜变性的机制。
Detachment of the neural retina from the pigment epithelium may be associated with tissue edema; however, the mechanisms of fluid accumulation are not understood. Because retinal detachment is usually not accompanied by vascular leakage, we investigated whether the osmotic swelling characteristics of retinal glial (Müller) cells are changed after experimental detachment of the porcine retina. Osmotic stress, induced by application of a hypotonic bath solution to retinal slices, caused swelling of Müller cell bodies in 7-day-detached retinas, but no swelling was inducible in slices of control retinas. Müller cell somata in slices of retinal areas that surround local detachment in situ also showed osmotic swelling, albeit at a smaller amplitude. The amplitude of osmotic Müller cell swelling correlated with the decrease in the K+ conductance, suggesting a causal relationship between both gliotic alterations. Further factors implicated in Müller cell swelling were inflammatory mediators and oxidative stress. We propose that a dysregulation of the ion and water transport through Müller cells may impair the fluid absorption from the retinal tissue, resulting in chronic fluid accumulation after detachment. This knowledge may lead to a better understanding of the mechanisms involved in retinal degeneration after detachment.
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分离的猫视网膜中的氨基酸特征。
DOI：
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发表时间：
1998
期刊：
Investigative ophthalmology & visual science.
影响因子：
--
作者：
Marc,RE;Murry,RF;Fisher,SK;Linberg,KA;Lewis,GP
通讯作者：
Lewis,GP
影响因子：
4.2
作者：
Mervin, K;Valter, K;Stone, J
通讯作者：
Stone, J
影响因子：
56.9
作者：
PAULSON, OB;NEWMAN, EA
通讯作者：
NEWMAN, EA
影响因子：
4.4
作者：
G. Lewis;K. Linberg;S. Fisher
通讯作者：
G. Lewis;K. Linberg;S. Fisher
P2Y(2) 受体激动剂 INS37217 对成年兔实验性视网膜脱离和视网膜电图的影响。
影响因子：
4.4
作者：
Meyer,CarstenH;Hotta,Kazuki;Peterson,WardM;Toth,CynthiaA;Jaffe,GlennJ
通讯作者：
Jaffe,GlennJ