Toxoplasma gondii actively remodels the microtubule network in host cells.
Toxoplasma gondii actively remodels the microtubule network in host cells.
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DOI：
10.1016/j.micinf.2008.08.014
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发表时间：
2008-11
影响因子：
5.8
通讯作者：
Hager KM
中科院分区：
文献类型：
--
作者：
Walker ME;Hjort EE;Smith SS;Tripathi A;Hornick JE;Hinchcliffe EH;Archer W;Hager KM
刚地弓形虫感染触发宿主微管重排和细胞器在寄生虫液泡周围的招募。影响微管重构初始阶段的因素尚不清楚。为了阐明这一机制，我们测试了寄生虫主动重塑宿主微管的假设。利用热杀寄生虫和延时分析，我们确定微管重排需要活的寄生虫，并且是时间依赖的。我们在感染后1小时内发现了一种新的微管（MTs）与液泡相关。这种aster缺少通常与MT成核位点相关的γ (γ)-微管蛋白的集中病灶。出乎意料的是，液泡增大与液泡周围MT染色的增加无关。我们得出结论，微管重构不是由空间约束引起的。通过nocodazole洗脱研究，我们证明液泡核通过γ-微管蛋白相关位点在体内促进宿主微管生长。此外，超感染的宿主细胞显示多个γ-微管蛋白灶。微管动力学对未感染细胞的细胞周期控制至关重要。利用非融合单层细胞，我们发现宿主细胞通常不能完成细胞分裂，从而产生更大的多核细胞。我们的数据表明，弓形虫与宿主微管之间的密切相互作用导致细胞分裂受到抑制和/或导致有丝分裂缺陷，从而为寄生虫复制提供了更大的空间。
Toxoplasma gondii infection triggers host microtubule rearrangement and organelle recruitment around the parasite vacuole. Factors affecting initial stages of microtubule remodeling are unknown. To illuminate the mechanism, we tested the hypothesis that the parasite actively remodels host microtubules. Utilizing heat-killed parasites and time-lapse analysis, we determined microtubule rearrangement requires living parasites and is time dependent. We discovered a novel aster of microtubules (MTs) associates with the vacuole within 1 h of infection. This aster lacks the concentrated foci of gamma (γ)-tubulin normally associated with MT nucleation sites. Unexpectedly, vacuole enlargement does not correlate with an increase in MT staining around the vacuole. We conclude microtubule remodeling does not result from steric constraints. Using nocodazole washout studies, we demonstrate the vacuole nucleates host microtubule growth in-vivo via γ-tubulin-associated sites. Moreover, superinfected host cells display multiple γ-tubulin foci. Microtubule dynamics are critical for cell cycle control in uninfected cells. Using non-confluent monolayers, we show host cells commonly fail to finish cytokinesis resulting in larger, multinucleated cells. Our data suggest intimate interactions between T. gondii and host microtubules result in suppression of cell division and/or cause a mitotic defect, thus providing a larger space for parasite duplication.
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DOI：
10.1187/cbe.05-02-0064
发表时间：
2005-01-01
期刊：
Cell biology education
影响因子：
--
作者：
Hinchcliffe, Edward H
通讯作者：
Hinchcliffe, Edward H
影响因子：
1.5
作者：
DEMELO, EJT;DECARVALHO, TU;DESOUZA, W
通讯作者：
DESOUZA, W
影响因子：
64.8
作者：
Saeij, J. P. J.;Coller, S.;Boothroyd, J. C.
通讯作者：
Boothroyd, J. C.
DOI：
10.1083/jcb.142.3.775
发表时间：
1998-08-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Moritz M;Zheng Y;Alberts BM;Oegema K
通讯作者：
Oegema K
影响因子：
64.5
作者：
Coppens, I;Dunn, JD;Joiner, KA
通讯作者：
Joiner, KA