The cauliflower mosaic virus (CaMV) 35S promoter sequence alters the level and patterns of activity of adjacent tissue- and organ-specific gene promoters
The cauliflower mosaic virus (CaMV) 35S promoter sequence alters the level and patterns of activity of adjacent tissue- and organ-specific gene promoters
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花椰菜花叶病毒 (CaMV) 35S 启动子序列改变邻近组织和器官特异性基因启动子的活性水平和模式
DOI：
10.1007/s00299-007-0307-x
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发表时间：
2007-03
期刊：
影响因子：
--
通讯作者：
Xuelian Zheng, Wei Deng,Yan Pei et. al.
中科院分区：
文献类型：
--
作者：
Xuelian Zheng, Wei Deng,Yan Pei et. al.
在这里，我们报告了 35S 启动子序列对烟草植物组织和器官特异性基因启动子活性的影响。在缺乏 35S 启动子序列的情况下，AAP2 启动子仅在维管组织中具有活性，如 AAP2:GUS 基因的表达所示。由于同一T质粒中的35S启动子序列，转基因植物的AAP2启动子活性增加了两倍至五倍，并且该启动子在所有组织类型中都变得活跃。含有子房特异性 AGL5:iaaM 基因（iaaM 编码生长素生物合成基因）的转基因植物除了产生无籽果实外，表现出野生型表型，而含有 AGL5:iaaM 基因和 35S 启动子序列的植物则显示出剧烈的形态变化。 RT-PCR分析证实该表型是由根、茎和花等非子房器官中AGL5:iaaM基因的激活引起的。当花粉、胚珠和早期胚胎特异性 PAB5:芽孢杆菌RNA酶基因（编码 RNase 基因的芽孢杆菌RNA酶）被转化时，35S启动子序列的存在大大降低了转化效率。然而，在没有35S启动子的情况下，转化效率恢复，表明35S启动子可能激活非生殖器官（例如愈伤组织和芽原基）中PAB5:barnase的表达。此外，如果35S启动子序列被NOS启动子序列替换，则没有观察到AAP2、AGL5或PAB5启动子活性的改变。我们的结果表明，35S启动子序列可以将邻近的组织和器官特异性基因启动子转化为全局活性启动子。
Here we report the effect of the 35S promoter sequence on activities of the tissue- and organ-specific gene promoters in tobacco plants. In the absence of the 35S promoter sequence the AAP2 promoter is active only in vascular tissues as indicated by expression of the AAP2:GUS gene. With the 35S promoter sequence in the same T-plasmid, transgenic plants exhibit twofold to fivefold increase in AAP2 promoter activity and the promoter becomes active in all tissue types. Transgenic plants hosting the ovary-specific AGL5:iaaM gene (iaaM coding an auxin biosynthetic gene) showed a wild-type phenotype except production of seedless fruits, whereas plants hosting the AGL5:iaaM gene along with the 35S promoter sequence showed drastic morphological alterations. RT-PCR analysis confirms that the phenotype was caused by activation of the AGL5:iaaM gene in non-ovary organs including roots, stems and flowers. When the pollen-, ovule- and early embryo-specific PAB5:barnase gene (barnase coding a RNase gene) was transformed, the presence of 35S promoter sequence drastically reduced transformation efficiencies. However, the transformation efficiencies were restored in the absence of 35S promoter, indicating that the 35S promoter might activate the expression of PAB5:barnase in non-reproductive organs such as calli and shoot primordia. Furthermore, if the 35S promoter sequence was replaced with the NOS promoter sequence, no alteration in AAP2, AGL5 or PAB5 promoter activities was observed. Our results demonstrate that the 35S promoter sequence can convert an adjacent tissue- and organ-specific gene promoter into a globally active promoter.
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影响因子：
5.1
作者：
BATTRAW, MJ;HALL, TC
通讯作者：
HALL, TC
影响因子：
4.6
作者：
E. Graaff;A. Dulk-Ras;P. Hooykaas;B. Keller
通讯作者：
E. Graaff;A. Dulk-Ras;P. Hooykaas;B. Keller
DOI：
10.1002/j.1460-2075.1987.tb02730.x
发表时间：
1987-12
期刊：
The EMBO Journal
影响因子：
--
作者：
R. Jefferson;T. Kavanagh;M. Bevan
通讯作者：
R. Jefferson;T. Kavanagh;M. Bevan
影响因子：
56.9
作者：
HAYASHI, H;CZAJA, I;WALDEN, R
通讯作者：
WALDEN, R
影响因子：
4.3
作者：
Ouwerkerk, PBF;de Kam, RJ;Meijer, AH
通讯作者：
Meijer, AH