Integrative and Comprehensive Pan-Cancer Analysis of Lymphocyte-Specific Protein Tyrosine Kinase in Human Tumors.
Integrative and Comprehensive Pan-Cancer Analysis of Lymphocyte-Specific Protein Tyrosine Kinase in Human Tumors.
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DOI：
10.3390/ijms232213998
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发表时间：
2022-11-13
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
淋巴细胞特异性蛋白酪氨酸激酶（LCK）常见于多种恶性血液病，但在实体瘤中相对不常见。本研究旨在通过整合和全面的泛癌分析以及实验验证，探索LCK在肿瘤中的潜在诊断和预后价值。利用多个数据库来探讨LCK在泛癌中的表达、变化、预后价值、与免疫浸润的关联以及潜在的功能途径。通过患者样品以及肿瘤细胞系的蛋白质印迹和qPCR进一步验证结果。LCK高表达通常代表较好的预后。值得注意的是，LCK的药物敏感性预测将P-529确定为药物开发的候选者。基因注释（GO）和KEGG分析显示PD-L1和T细胞受体途径的显著富集。患者样本和肿瘤细胞系的结果证实了LIHC中的这些结论。总之，LCK在多种肿瘤和正常组织中差异表达。进一步的分析强调了其与预后影响、泛癌遗传改变和免疫特征的关联。我们的数据为LCK的诊断标志物和LCK作为肿瘤治疗靶点的可能用途提供了证据。
Lymphocyte-specific protein tyrosine kinase (LCK) is common in a variety of hematologic malignancies but comparatively less common in solid tumors. This study aimed to explore the potential diagnostic and prognostic value of LCK across tumors through integrative and comprehensive pan-cancer analysis, as well as experimental validation. Multiple databases were used to explore the expression, alteration, prognostic value, association with immune infiltration, and potential functional pathways of LCK in pan-cancers. The results were further validated by western blotting and qPCR of patient samples as well as tumor cell lines. High LCK expression typically represents a better prognosis. Notably, drug sensitivity prediction of LCK identified P-529 as a candidate for drug development. Gene Annotations (GO) and KEGG analyses showed significant enrichment of PD-L1 and the T-cell receptor pathway. The results from patient samples and tumor cell lines confirmed these conclusions in LIHC. In conclusion, LCK is differentially expressed in multiple tumors and normal tissues. Further analysis highlighted its association with prognostic implications, pan-cancer genetic alterations, and immune signatures. Our data provide evidence for a diagnostic marker of LCK and the possible use of LCK as a target for the treatment of tumors.
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影响因子：
3.1
作者：
Gucalp A;Sparano JA;Caravelli J;Santamauro J;Patil S;Abbruzzi A;Pellegrino C;Bromberg J;Dang C;Theodoulou M;Massague J;Norton L;Hudis C;Traina TA
通讯作者：
Traina TA
影响因子：
7.3
作者：
Gao J;Aksoy BA;Dogrusoz U;Dresdner G;Gross B;Sumer SO;Sun Y;Jacobsen A;Sinha R;Larsson E;Cerami E;Sander C;Schultz N
通讯作者：
Schultz N
影响因子：
7.3
作者：
Dolina JS;Van Braeckel-Budimir N;Thomas GD;Salek-Ardakani S
通讯作者：
Salek-Ardakani S
影响因子：
4
作者：
Xing J;Yi J
通讯作者：
Yi J
影响因子：
3.4
作者：
Gangadhar, Tara C.;Clark, Joseph I.;Gajewski, Thomas F.
通讯作者：
Gajewski, Thomas F.