Transcriptional profiles of non-neuronal and immune cells in mouse trigeminal ganglia.
Transcriptional profiles of non-neuronal and immune cells in mouse trigeminal ganglia.
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DOI：
10.3389/fpain.2023.1274811
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发表时间：
2023
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影响因子：
--
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中科院分区：
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非神经元细胞占感觉神经节的90%-95%。这些细胞，特别是神经胶质细胞和免疫细胞，在感觉神经元的调节中起着关键作用。本研究旨在使用已发表的和我们自己的单细胞RNA测序、流式细胞术和免疫组织化学生成的数据，识别、分析和总结幼稚雄性小鼠三叉神经节（TG）非神经元细胞的类型。TG具有五种类型的非神经元细胞，即神经胶质细胞、成纤维细胞、平滑肌细胞、内皮细胞和免疫细胞。神经胶质细胞、成纤维细胞、平滑肌和内皮细胞的出版物之间存在一致性。根据基因谱，神经胶质细胞分为有髓和无髓雪旺细胞和卫星神经胶质细胞。Mpz在雪旺细胞中具有优势表达，Fabp 7对SCG具有特异性。两种类型的Col 1a 2+成纤维细胞位于整个TG的区别。使用明确定义的标记物检测血管中的TG平滑肌和内皮细胞。我们的研究报告了三种类型的巨噬细胞（Mph）和四种类型的中性粒细胞（Neu）在TG。Mph位于神经元体和神经纤维中，并通过以Ccr 2、Cx 3cr 1和Iba 1为标记物的独特转录组学图谱进行亚组化。数据库的比较显示，1型Mph与脉络丛低（CPlo）边界相关Mph（BAM）相似。2型Mph对CPhi BAM的预测得分最高，而3型Mph则不同。S100 a8 + Neu定位于TG周围硬脑膜，根据Csf 3r、Ly 6 G、Ngp、Elane和Mpo的表达和聚类情况进行分组。对已发表数据集的综合分析表明，Neu-1、Neu-2和Neu-3与脑Neu-1组相似，而Neu-4与单核细胞衍生细胞相似。总体而言，生成和总结的非神经元TG细胞数据集显示了TG中髓样细胞类型的独特组成，可以为细胞可塑性、神经元和非神经元细胞之间的相互作用网络以及头颈部各种疼痛条件下的功能研究提供必要和基本的信息。
Non-neuronal cells constitute 90%–95% of sensory ganglia. These cells, especially glial and immune cells, play critical roles in the modulation of sensory neurons. This study aimed to identify, profile, and summarize the types of trigeminal ganglion (TG) non-neuronal cells in naïve male mice using published and our own data generated by single-cell RNA sequencing, flow cytometry, and immunohistochemistry. TG has five types of non-neuronal cells, namely, glial, fibroblasts, smooth muscle, endothelial, and immune cells. There is an agreement among publications for glial, fibroblasts, smooth muscle, and endothelial cells. Based on gene profiles, glial cells were classified as myelinated and non-myelinated Schwann cells and satellite glial cells. Mpz has dominant expression in Schwann cells, and Fabp7 is specific for SCG. Two types of Col1a2+ fibroblasts located throughout TG were distinguished. TG smooth muscle and endothelial cells in the blood vessels were detected using well-defined markers. Our study reported three types of macrophages (Mph) and four types of neutrophils (Neu) in TG. Mph were located in the neuronal bodies and nerve fibers and were sub-grouped by unique transcriptomic profiles with Ccr2, Cx3cr1, and Iba1 as markers. A comparison of databases showed that type 1 Mph is similar to choroid plexus-low (CPlo) border-associated Mph (BAMs). Type 2 Mph has the highest prediction score with CPhi BAMs, while type 3 Mph is distinct. S100a8+ Neu were located in the dura surrounding TG and were sub-grouped by clustering and expressions of Csf3r, Ly6G, Ngp, Elane, and Mpo. Integrative analysis of published datasets indicated that Neu-1, Neu-2, and Neu-3 are similar to the brain Neu-1 group, while Neu-4 has a resemblance to the monocyte-derived cells. Overall, the generated and summarized datasets on non-neuronal TG cells showed a unique composition of myeloid cell types in TG and could provide essential and fundamental information for studies on cell plasticity, interactomic networks between neurons and non-neuronal cells, and function during a variety of pain conditions in the head and neck regions.
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影响因子：
6
作者：
Domoto R;Sekiguchi F;Tsubota M;Kawabata A
通讯作者：
Kawabata A
影响因子：
32.4
作者：
Guilliams M;Dutertre CA;Scott CL;McGovern N;Sichien D;Chakarov S;Van Gassen S;Chen J;Poidinger M;De Prijck S;Tavernier SJ;Low I;Irac SE;Mattar CN;Sumatoh HR;Low GHL;Chung TJK;Chan DKH;Tan KK;Hon TLK;Fossum E;Bogen B;Choolani M;Chan JKY;Larbi A;Luche H;Henri S;Saeys Y;Newell EW;Lambrecht BN;Malissen B;Ginhoux F
通讯作者：
Ginhoux F
影响因子：
16.6
作者：
Avraham O;Deng PY;Jones S;Kuruvilla R;Semenkovich CF;Klyachko VA;Cavalli V
通讯作者：
Cavalli V
影响因子：
4.8
作者：
Chu, Yanhao;Jia, Shilin;Xu, Ke;Liu, Qing;Mai, Lijia;Liu, Jiawei;Fan, Wenguo;Huang, Fang
通讯作者：
Huang, Fang
影响因子：
16.6
作者：
通讯作者：
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