Click chemistry-mediated cyclic cleavage of metal ion-dependent DNAzymes for amplified and colorimetric detection of human serum copper (II)
Click chemistry-mediated cyclic cleavage of metal ion-dependent DNAzymes for amplified and colorimetric detection of human serum copper (II)
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点击化学介导的金属离子依赖性脱氧核糖核酸酶的循环裂解，用于人血清铜的扩增和比色检测 (II)
DOI：
10.1007/s00216-017-0587-0
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发表时间：
2017-09
影响因子：
4.3
通讯作者：
Xiang Yun
中科院分区：
文献类型：
--
作者：
Li Daxiu;Xie Jiaqing;Zhou Wenjiao;Jiang Bingying;Yuan Ruo;Xiang Yun
Cu2+水平的测定在疾病诊断和环境监测中具有重要作用。通过Cu+催化的点击化学和金属离子依赖的DNAzyme循环扩增的耦合作用，我们建立了一种简便、灵敏的检测人血清中Cu2+的比色传感方法。靶Cu2+可以被抗坏血酸还原成Cu+， Cu+催化叠氮化物和炔修饰dna之间的叠氮化物-炔环加成，形成Mg2+依赖性DNAzymes。随后，Mg2+离子催化DNAzymes的发夹DNA底物序列的裂解，触发大量游离g -四重体序列的循环生成，这些游离g -四重体序列与hemin结合形成g -四重体/hemin人工过氧化物酶，使感应溶液发生明显的色变，用于灵敏比色检测Cu2+。该方法对Cu2+的检测动态范围为5 ~ 500 nM，检测限为2 nM。此外，还可以利用这种传感方法测定人血清中Cu2+的水平。该传感方法具有简便、灵敏度高的优点，对不同样品中Cu2+的现场测定具有很大的潜力。图形化文摘：金属离子依赖性dnazymes与咔嗒化学耦合的灵敏比色法检测铜（II）
The determination of the level of Cu2+plays important roles in disease diagnosis and environmental monitoring. By coupling Cu+-catalyzed click chemistry and metal ion-dependent DNAzyme cyclic amplification, we have developed a convenient and sensitive colorimetric sensing method for the detection of Cu2+in human serums. The target Cu2+can be reduced by ascorbate to form Cu+, which catalyzes the azide-alkyne cycloaddition between the azide- and alkyne-modified DNAs to form Mg2+-dependent DNAzymes. Subsequently, the Mg2+ions catalyze the cleavage of the hairpin DNA substrate sequences of the DNAzymes and trigger cyclic generation of a large number of free G-quadruplex sequences, which bind hemin to form the G-quadruplex/hemin artificial peroxidase to cause significant color transition of the sensing solution for sensitive colorimetric detection of Cu2+. This method shows a dynamic range of 5 to 500 nM and a detection limit of 2 nM for Cu2+detection. Besides, the level of Cu2+in human serums can also be determined by using this sensing approach. With the advantages of simplicity and high sensitivity, such sensing method thus holds great potential for on-site determination of Cu2+in different samples.Graphical abstractSensitive colorimetric detection of copper (II) by coupling click chemistry with metal ion-dependentDNAzymes
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影响因子：
16.6
作者：
Qu, Weisi;Liu, Yingyi;Jiang, Xingyu
通讯作者：
Jiang, Xingyu
影响因子：
62.1
作者：
Liu, Juewen;Cao, Zehui;Lu, Yi
通讯作者：
Lu, Yi
影响因子：
--
作者：
BREAKER, RR;JOYCE, GF
通讯作者：
JOYCE, GF
使用点击化学通过商用血糖仪灵敏检测铜 (II)
影响因子：
12.6
作者：
Su, Jiao;Xu, Jin;Chai, Yaqin
通讯作者：
Chai, Yaqin
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
Zhi Shan;Mingsheng Lu;Li Wang;Bruce MacDonald;Judy MacInnis;M. Mkandawire;K. Oakes;Xu Zhang
通讯作者：
Zhi Shan;Mingsheng Lu;Li Wang;Bruce MacDonald;Judy MacInnis;M. Mkandawire;K. Oakes;Xu Zhang