Use of coated silver nanoparticles to understand the relationship of particle dissolution and bioavailability to cell and lung toxicological potential.
Use of coated silver nanoparticles to understand the relationship of particle dissolution and bioavailability to cell and lung toxicological potential.
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DOI：
10.1002/smll.201301597
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发表时间：
2014-01-29
期刊：
影响因子：
13.3
通讯作者：
Nel, Andre E.
中科院分区：
文献类型：
--
作者：
Wang, Xiang;Ji, Zhaoxia;Chang, Chong Hyun;Zhang, Haiyuan;Wang, Meiying;Liao, Yu-Pei;Lin, Sijie;Meng, Huan;Li, Ruibin;Sun, Bingbing;Winkle, Laura Van;Pinkerton, Kent E.;Zink, Jeffrey I.;Xia, Tian;Nel, Andre E.
由于超过30%的包含工程纳米材料的消费品含有纳米银，这种材料的安全性受到公众的极大关注。在这项研究中，我们使用银纳米颗粒（NP）来证明，20 nm聚乙烯吡咯烷酮（PVP或P）和柠檬酸盐（C）涂层的银纳米颗粒诱导更多的细胞毒性和氧化应激比更大（110 nm）的颗粒，由于更高的溶解速率和银的生物利用度。此外，与C110相比，柠檬酸盐20 nm（C20）纳米颗粒在肺中产生急性嗜酸性炎症和产生趋化因子的倾向也更高。P110的细胞毒性效应低于C110，可能是由于PVP能够络合释放的Ag+。与C20更强烈的急性肺效应相反，C110在第21天诱导轻度肺纤维化，可能是由于缓慢但持续的Ag+释放导致亚慢性损伤反应。有趣的是，释放的金属Ag被掺入沉积在气道和肺纤维周围的胶原纤维中。总之，这些结果表明，尺寸和表面涂层影响Ag纳米颗粒的细胞毒性以及它们的急性与亚慢性肺损伤潜力。
Since more than 30 % of consumer products that include engineered nanomaterials contain nano-Ag, the safety of this material is of considerable public concern. In this study, we used Ag nanoparticles (NPs) to demonstrate that 20 nm polyvinylpyrrolidone (PVP or P) and citrate (C)-coated Ag NPs induce more cellular toxicity and oxidative stress than larger (110 nm) particles due to a higher rate of dissolution and Ag bioavailability. Moreover, there was also a higher propensity for citrate 20 nm (C20) nanoparticles to generate acute neutrophilic inflammation in the lung and to produce chemokines compared to C110. P110 had less cytotoxic effects than C110, likely due to the ability of PVP to complex released Ag+. In contrast to the more intense acute pulmonary effects of C20, C110 induced mild pulmonary fibrosis at day 21, likely as a result of slow but persistent Ag+ release leading to a sub-chronic injury response. Interestingly, the released metallic Ag gets incorporated into the collagen fibers depositing around airways and the lung interstitium. Taken together, these results demonstrate that size and surface coating affect the cellular toxicity of Ag NPs as well as their acute versus sub-chronic lung injury potential.
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影响因子：
10
作者：
Hamilton RF;Wu N;Porter D;Buford M;Wolfarth M;Holian A
通讯作者：
Holian A
影响因子：
17.1
作者：
George, Saji;Pokhrel, Suman;Xia, Tian;Gilbert, Benjamin;Ji, Zhaoxia;Schowalter, Marco;Rosenauer, Andreas;Damoiseaux, Robert;Bradley, Kenneth A.;Maedler, Lutz;Nel, Andre E.
通讯作者：
Nel, Andre E.
DOI：
10.1006/faat.1995.1022
发表时间：
1995-02-01
期刊：
FUNDAMENTAL AND APPLIED TOXICOLOGY
影响因子：
--
作者：
HENDERSON, RF;DRISCOLL, KE;BARR, EB
通讯作者：
BARR, EB
影响因子：
3.5
作者：
Hyun, Jin-Sook;Lee, Byoung Seok;Yu, Il Je
通讯作者：
Yu, Il Je
影响因子：
1.7
作者：
OBERDORSTER, G;FERIN, J;MORROW, PE
通讯作者：
MORROW, PE