Massive gene transfer and extensive RNA editing of a symbiotic dinoflagellate plastid genome.
Massive gene transfer and extensive RNA editing of a symbiotic dinoflagellate plastid genome.
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DOI：
10.1093/gbe/evu109
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发表时间：
2014-05-31
影响因子：
3.3
通讯作者：
Shoguchi E
中科院分区：
文献类型：
--
作者：
Mungpakdee S;Shinzato C;Takeuchi T;Kawashima T;Koyanagi R;Hisata K;Tanaka M;Goto H;Fujie M;Lin S;Satoh N;Shoguchi E
微小共生藻的基因组测序结果表明，109个质体相关基因中有95个已转移到核基因组中，随后通过基因复制进行扩增。只有14个基因保留在质体中，并以DNA微环的形式存在。每个小环（1.8-3.3 kb）含有一个基因和一个保守的非编码区，该非编码区含有推定的启动子和RNA结合位点。9种类型的RNA编辑，包括一种新的G/U类型，在小环转录本中发现，但在转移到细胞核的基因中没有发现。与甲藻线粒体中的DNA编辑位点相反，其在所有分类群中往往是高度保守的，DNA微环中使用的编辑位点在物种之间是高度可变的。编辑对于核心光系统蛋白功能至关重要。它恢复进化上保守的氨基酸和增加肽基亲水性。它还增加启动光系统复合物组装所必需的蛋白质可塑性。
Genome sequencing of Symbiodinium minutum revealed that 95 of 109 plastid-associated genes have been transferred to the nuclear genome and subsequently expanded by gene duplication. Only 14 genes remain in plastids and occur as DNA minicircles. Each minicircle (1.8–3.3 kb) contains one gene and a conserved noncoding region containing putative promoters and RNA-binding sites. Nine types of RNA editing, including a novel G/U type, were discovered in minicircle transcripts but not in genes transferred to the nucleus. In contrast to DNA editing sites in dinoflagellate mitochondria, which tend to be highly conserved across all taxa, editing sites employed in DNA minicircles are highly variable from species to species. Editing is crucial for core photosystem protein function. It restores evolutionarily conserved amino acids and increases peptidyl hydropathy. It also increases protein plasticity necessary to initiate photosystem complex assembly.
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影响因子：
9.2
作者：
Hackett, JD;Yoon, HS;Bhattacharya, D
通讯作者：
Bhattacharya, D
影响因子：
3.4
作者：
Hoffman GE;Sanchez Puerta MV;Delwiche CF
通讯作者：
Delwiche CF
影响因子：
4.4
作者：
Barbrook AC;Santucci N;Plenderleith LJ;Hiller RG;Howe CJ
通讯作者：
Howe CJ
影响因子：
10.7
作者：
Janouskovec, Jan;Sobotka, Roman;Keeling, Patrick J.
通讯作者：
Keeling, Patrick J.
影响因子：
5.1
作者：
Barbrook, Adrian C.;Dorrell, Richard G.;Howe, Christopher J.
通讯作者：
Howe, Christopher J.