ABO, D blood typing and subtyping using plug-based microfluidics.
ABO, D blood typing and subtyping using plug-based microfluidics.
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DOI：
10.1021/ac800485q
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发表时间：
2008-08-15
影响因子：
7.4
通讯作者：
Ismagilov, Rustem F.
中科院分区：
文献类型：
--
作者：
Kline, Timothy R.;Runyon, Matthew K.;Pothiawala, Mohammad;Ismagilov, Rustem F.
使用基于塞的微流体方法平行进行多个凝集测定，而没有交叉污染，并且仅使用微升体积的血液。为了在芯片上进行凝集测定，设计微流体装置以将抗体、缓冲液和红细胞（RBC）的水性流联合收割机组合以形成被氟化载体流体包围的体积为30-40 nL的液滴。使用这种方法，通过对多种抗原进行筛选而没有交叉污染，成功地进行了ABO和D（Rh）血型定型和A组亚型定型的概念验证。通过使用基于凝集素的稀释试验，芯片上亚型分型区分常见的A1和A2 RBC。通过分析凝集亲合力作为剪切速率的函数，该灵活的平台被扩展以区分罕见的弱凝集的A亚型RBC。塞内对比度变化的定量分析揭示了凝集动力学的微妙之处，并能够表征罕见血液亚型的凝集。最后，该平台用于检测细菌，证明了该检测方法在检测败血症方面的潜在有用性以及在基于凝集的病毒检测中应用的潜力。该技术提供的速度、控制和最小样本消耗为护理点应用、新生儿血型鉴定和小型模式生物体中的一般血液测定带来了进步。
A plug-based microfluidic approach was used to perform multiple agglutination assays in parallel without cross-contamination and using only microliter volumes of blood. To perform agglutination assays on-chip, a microfluidic device was designed to combine aqueous streams of antibody, buffer, and red blood cells (RBCs) to form droplets 30–40 nL in volume surrounded by a fluorinated carrier fluid. Using this approach, proof-of-concept ABO and D (Rh) blood typing and group A subtyping were successfully performed by screening against multiple antigens without cross-contamination. On-chip subtyping distinguished common A1 and A2 RBCs by using a lectinbased dilution assay. This flexible platform was extended to differentiate rare, weakly agglutinating RBCs of A subtypes by analyzing agglutination avidity as a function of shear rate. Quantitative analysis of changes in contrast within plugs revealed subtleties in agglutination kinetics and enabled characterization of agglutination of rare blood subtypes. Finally, this platform was used to detect bacteria, demonstrating the potential usefulness of this assay in detecting sepsis and the potential for applications in agglutination-based viral detection. The speed, control, and minimal sample consumption provided by this technology present an advance for point of care applications, blood typing of newborns, and general blood assays in small model organisms.
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影响因子：
1.7
作者：
Avent, Neil D.
通讯作者：
Avent, Neil D.
影响因子：
6.2
作者：
Brito, VO;Raimundo, IM
通讯作者：
Raimundo, IM
DOI：
10.1073/pnas.0507171102
发表时间：
2006-01-17
影响因子：
11.1
作者：
Abkarian, M;Faivre, M;Stone, HA
通讯作者：
Stone, HA
影响因子：
16.6
作者：
Song, H;Tice, JD;Ismagilov, RF
通讯作者：
Ismagilov, RF
影响因子：
7.4
作者：
Liau, A;Karnik, R;Cate, JHD
通讯作者：
Cate, JHD