Bioinformatics Analysis of circRNA Expression and Construction of "circRNA-miRNA-mRNA" Competing Endogenous RNAs Networks in Bipolar Disorder Patients.
Bioinformatics Analysis of circRNA Expression and Construction of "circRNA-miRNA-mRNA" Competing Endogenous RNAs Networks in Bipolar Disorder Patients.
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DOI：
10.3389/fgene.2021.718976
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发表时间：
2021
影响因子：
3.7
通讯作者：
Wei B
中科院分区：
文献类型：
--
作者：
Fu Y;He W;Zhou C;Fu X;Wan Q;He L;Wei B
双相情感障碍(BD)是中国的一种严重的心境障碍疾病，其发病机制尚不清楚。环状RNAs(CircRNAs)在精神障碍中起关键作用，可作为竞争性内源性RNAs(CeRNAs)。然而，对于CircRNAs与BD的相关性却知之甚少。在这项研究中，使用Deep RNA测序技术来鉴定BD患者和对照组之间差异表达的CircRNAs(DE-CircRNAs)和差异表达的mRNAs(DE-mRNAs)。采用实时定量逆转录聚合酶链式反应(qRT-PCR)验证差异表达RNA(DE-RNAs)。在两组标本中，共有9,593个CircRNAs和20030个mRNAs，其中50个DE-CircRNAs和244个DE-mRNAs显著上调，44个DE-CircRNAs和294个DE-mRNAs显著下调。根据ceRNAs的调控机制，CircRNAs可以直接结合microRNAs(MiRNAs)来影响mRNA的表达，并且CircRNAs和mRNAs的表达趋势是一致的。根据这一机制，我们利用RNA测序数据构建了两个CENA网络。通过富集分析进一步阐明了这些DE-CircRNAs的功能。综上所述，本研究表明BD患者的CircRNA表达谱发生了改变，构建了CERNA调控网络，为BD的发病机制提供了假说。
Bipolar disorder (BD) is a severe mood disorder disease in China, and its underlying pathogenesis remains unknown. Circular RNAs (circRNAs) have been reported to play a key role in mental disorders and can be used as competitive endogenous RNAs (ceRNAs). However, little is known about the correlation of circRNAs with BD. In this study, Deep RNA sequencing was used to identify differentially expressed circRNAs (DE-circRNAs) and differentially expressed mRNAs (DE-mRNAs) between BD patients and a control group. Real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to validate the differentially expressed RNAs (DE-RNAs). In all 9,593 circRNAs and 20,030 mRNAs were found in the two groups of specimens, among which 50 DE-circRNAs and 244 DE-mRNAs were significantly upregulated, and 44 DE-circRNAs and 294 DE-mRNAs were significantly downregulated. Based on the regulatory mechanism of ceRNAs, circRNAs can directly bind microRNAs (miRNAs) to affect mRNA expression, and the expression trends of circRNAs and mRNAs are consistent. According to this mechanism, we constructed two ceRNA networks by using the RNA sequencing data. The function of these DE-circRNAs was further elucidated by enrichment analysis. In summary, the present study showed that the circRNA expression profile of BD patients is altered, and a ceRNA regulatory network was constructed, which provided a hypothesis about the pathogenesis of BD.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
10.6
作者：
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通讯作者：
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影响因子：
5.1
作者：
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DOI：
10.1111/j.1749-6632.2012.06786.x
发表时间：
2013-01-01
期刊：
TRANSLATIONAL IMMUNOLOGY IN ASIA-OCEANIA
影响因子：
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作者：
Qian, Cheng;Cao, Xuetao
通讯作者：
Cao, Xuetao
影响因子：
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作者：
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通讯作者：
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