QCM Analysis for Two-Step Adsorption of Albumin and Fibronectin on Zirconia Surface
QCM Analysis for Two-Step Adsorption of Albumin and Fibronectin on Zirconia Surface
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白蛋白和纤连蛋白在氧化锆表面两步吸附的 QCM 分析
DOI：
10.1155/2021/2492387
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发表时间：
2021
影响因子：
--
通讯作者：
Hayakawa Tohru
中科院分区：
文献类型：
--
作者：
Hirota Masatsugu;Hayakawa Tohru
蛋白质在牙种植体表面的吸附是骨整合的第一步，起着关键作用。许多类型的蛋白质存在于活体中，并竞争吸附在材料表面上。作为植入材料，部分稳定氧化锆（ZrO 2）目前是钛的有吸引力的替代品，可克服钛植入物的缺点。采用27 MHz石英晶体微天平（QCM）法研究了纤连蛋白（Fn）和牛血清白蛋白（Alb）在ZrO 2表面的两步吸附。采用ZrO 2传感器进行QCM测量。如下进行两步吸附。(1)Fn‐Alb系列：首先将Fn溶液注入磷酸盐缓冲盐水（PBS）溶液中，然后注入Alb溶液。(2)Alb‐Fn系列：首先注入Alb溶液，然后注入Fn溶液。在每次蛋白质注射后监测频率降低60分钟。通过观察频率的降低来计算Fn或Alb的吸附量，并且通过频率偏移对吸附时间的曲线拟合来获得表观反应速率kobs。Fn-Alb和Alb-Fn系列之间Fn和Alb的吸附量无显著差异（P> 0.05）。第二步吸附Fn和Alb的速率Thekobs均显著低于第一步吸附（P< 0.05）。ZrO 2传感器的表面形貌与蛋白质的吸附量之间没有明显的相关性。结果表明，蛋白质在ZrO 2表面的吸附量不受两步吸附系列的影响，但第二步吸附速率受第一步吸附的影响。
The adsorption of proteins on the dental implant surface is the first step in the key role of osseointegration. Many types of proteins exist in the living body and compete for adsorption on the material surface. As an implant material, partially stabilized zirconia (ZrO2) is currently an attractive alternative to titanium to overcome the shortcomings of titanium implants. In this study, we investigated the two‐step adsorption of fibronectin (Fn) and bovine serum albumin (Alb) on the ZrO2surface using a 27‐MHz quartz crystal microbalance (QCM) method. A ZrO2sensor was employed for the QCM measurements. Two‐step adsorptions were performed as follows. (1) Fn‐Alb series: first, the Fn solution was injected into the phosphate‐buffered saline (PBS) solution, followed by the Alb solution. (2) Alb‐Fn series: first, the Alb solution was injected, followed by the Fn solution. The decrease in frequency was monitored for 60 minutes after each protein injection. The adsorbed amounts of Fn or Alb were calculated by observing the decrease in frequency, and the apparent reaction rate,kobs, was obtained through the curve fitting of frequency shift against the adsorption time. No significant difference was observed in the adsorbed amounts of Fn and Alb between the Fn‐Alb and Alb‐Fn series (P> 0.05). Thekobs, rate of protein adsorption, in the second step was significantly slower than that in the first step for both Fn and Alb adsorption (P< 0.05). There was no clear correlation between the amount of protein adsorbed on the ZrO2sensor and the surface topography. It was concluded that the amount of protein adsorbed on the ZrO2surface was not influenced by the two‐step adsorption series, but the adsorption rate of proteins in the second step was affected by the first‐step protein adsorption.
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2. 表面科学与技术中的生物材料-组织界面及其类似物
DOI：
10.3138/9781442671508-005
发表时间：
1991
期刊：
The International journal of oral & maxillofacial implants
影响因子：
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作者：
B. Kasemo;J. Lausmaa
通讯作者：
J. Lausmaa
使用石英晶体微天平法分析蛋白质在钛表面上的两步吸附
DOI：
--
发表时间：
2020
期刊：
影响因子：
--
作者：
Hideki Imagami;Hideki Nomoto;T. Nomura;Shuichiro Maekawa;N. Mishima;Hideki Oikawa;Isao Ohashi;T. Hayakawa
通讯作者：
T. Hayakawa
纤连蛋白、纤维蛋白原和白蛋白在 TiO2 上的吸附：时间分辨动力学、结构变化和竞争研究
影响因子：
2.1
作者：
M. Pegueroles;C. Tonda;J. Planell;F. Gil;C. Aparicio
通讯作者：
C. Aparicio
影响因子：
5.8
作者：
Hirsh, Stacey L.;McKenzie, David R.;Bilek, Marcela M. M.
通讯作者：
Bilek, Marcela M. M.
蛋白质吸附表征。
影响因子：
--
作者：
M. Martins;Susana Sousa;Susana Sousa;J. Antunes;M. Barbosa
通讯作者：
M. Barbosa